PUBLICATION

A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance

Authors
Gabor, K., Charette, J., Pietraszewski, M., Wingfield, D., Shim, J., Millard, P., Kim, C.
ID
ZDB-PUB-150131-1
Date
2015
Source
Developmental and comparative immunology   51(2): 298-304 (Journal)
Registered Authors
Kim, Carol H.
Keywords
Danio rerio, Type I IFN, Zebrafish, antiviral, mda5, rhabdovirus
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • DEAD-box RNA Helicases/genetics
  • DEAD-box RNA Helicases/immunology
  • DEAD-box RNA Helicases/metabolism*
  • Immunity, Active/genetics
  • Interferon Type I/metabolism
  • Mutation/genetics
  • Receptors, Pattern Recognition/genetics
  • Receptors, Pattern Recognition/immunology
  • Receptors, Pattern Recognition/metabolism*
  • Rhabdoviridae/immunology*
  • Rhabdoviridae Infections/immunology*
  • Transgenes/genetics
  • Viral Load/genetics
  • Zebrafish/immunology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/immunology
  • Zebrafish Proteins/metabolism*
PubMed
25634485 Full text @ Dev. Comp. Immunol.
Abstract
Melanoma Differentiation-Associated protein 5 (MDA5) is a member of the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family, which are cytosolic pattern recognition receptors that detect viral nucleic acids. Here we show an Mda5-dependent response to rhabdovirus infection in vivo using a dominant-negative mda5 transgenic zebrafish. Dominant-negative mda5 zebrafish embryos displayed an impaired antiviral immune response compared to wild-type counterparts that can be rescued by recombinant full-length Mda5. To our knowledge, we have generated the first dominant-negative mda5 transgenic zebrafish and demonstrated a critical role for Mda5 in the antiviral response to rhabdovirus.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping