PUBLICATION
Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells
- Authors
- Qiao, J., Cui, S.J., Xu, L.L., Chen, S.J., Yao, J., Jiang, Y.H., Peng, G., Fang, C.Y., Yang, P.Y., Liu, F.
- ID
- ZDB-PUB-150113-7
- Date
- 2015
- Source
- Oncotarget 6(2): 1171-89 (Journal)
- Registered Authors
- Chen, Sijie, Liu, Feng, Peng, Gang
- Keywords
- none
- MeSH Terms
-
- Adult
- Aged
- Aged, 80 and over
- Cell Line, Tumor
- Cell Movement/genetics
- Cell Proliferation/genetics
- Chromatography, Liquid
- Female
- Filamins/genetics
- Filamins/metabolism*
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Male
- Mass Spectrometry
- Middle Aged
- Neoplasms/genetics
- Neoplasms/metabolism*
- Neoplasms/pathology
- Proteome/genetics
- Proteome/metabolism*
- Proteomics/methods*
- RNA Interference
- Reverse Transcriptase Polymerase Chain Reaction
- Stomach Neoplasms/genetics
- Stomach Neoplasms/metabolism*
- Stomach Neoplasms/pathology
- PubMed
- 25577646 Full text @ Oncotarget
Citation
Qiao, J., Cui, S.J., Xu, L.L., Chen, S.J., Yao, J., Jiang, Y.H., Peng, G., Fang, C.Y., Yang, P.Y., Liu, F. (2015) Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells. Oncotarget. 6(2):1171-89.
Abstract
Gastric cancer (GC) is the fourth and fifth most common cancer in men and women, respectively. We identified 2,750 proteins at false discovery rates of 1.3% (protein) and 0.03% (spectrum) by comparing the proteomic profiles of three GC and a normal gastric cell lines. Nine proteins were significantly dysregulated in all three GC cell lines, including filamin C, a muscle-specific filamin and a large actin-cross-linking protein. Downregulation of filamin C in GC cell lines and tissues were verified using quantitative PCR and immunohistochemistry. Data-mining using public microarray datasets shown that filamin C was significantly reduced in many human primary and metastasis cancers. Transient expression or silencing of filamin C affected the proliferation and colony formation of cancer cells. Silencing of endogenous filamin C enhanced cancer cell migration and invasion, whereas ectopic expression of filamin C had opposing effects. Silencing of filamin C increased the expression of matrix metallopeptidase 2 and improved the metastasis of prostate cancer in a zebrafish model. High filamin C associated with better prognosis of prostate cancer, leukemia and breast cancer patients. These findings establish a functional role of filamin C in human cancers and these data will be valuable for further study of its mechanisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping