PUBLICATION
Acute and Chronic Alcohol Administration: Effects on Performance of Zebrafish in a Latent Learning Task
- Authors
- Luchiari, A.C., Salajan, D.C., Gerlai, R.
- ID
- ZDB-PUB-150106-2
- Date
- 2015
- Source
- Behavioural brain research 282: 76-83 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- Alcohol, High-throughput screening, Latent learning, Memory, Zebrafish
- MeSH Terms
-
- Alcohol-Related Disorders/psychology*
- Animals
- Behavior, Animal/drug effects*
- Disease Models, Animal
- Drug Tolerance*
- Ethanol/administration & dosage
- Ethanol/pharmacology*
- Maze Learning/drug effects*
- Memory/drug effects*
- Time Factors
- Zebrafish
- PubMed
- 25557800 Full text @ Behav. Brain Res.
Citation
Luchiari, A.C., Salajan, D.C., Gerlai, R. (2015) Acute and Chronic Alcohol Administration: Effects on Performance of Zebrafish in a Latent Learning Task. Behavioural brain research. 282:76-83.
Abstract
Alcohol abuse is a major medical problem. Zebrafish have been proposed to model alcohol related human disorders. Alcohol impairs learning and memory. Here, we analyze the effects of alcohol on performance of zebrafish in a recently developed latent learning paradigm. We employ a 2 x 3 x 2 experimental design (chronic x acute alcohol treatment x path blocked). The latent learning task had two phases: one, 30min long exploration trials (16 days, 1 trial/day) with left or right path of a complex maze blocked, and two, a subsequent probe trial with all paths open leading to a goal box that now contained stimulus fish. During the 16 days each fish received one of two chronic treatments: freshwater or 0.50% (vol/vol%) alcohol. Subsequently, fish were immersed for 1h in one of the following solutions: 0.00 (freshwater), 0.50 or 1.00% alcohol, the acute challange. Behavior of fish was recorded during the probe trial that commenced immediately after the acute treatment. Path choices, latency to leave the start box and to enter the goal box, time spent in the goal box, distance travelled, and duration of freezing were quantified. We found that acute exposure to 1.00% alcohol after chronic freshwater disrupted learning performance, so did exposure to freshwater after chronic alcohol treatment (withdrawal). We also found exposure to chronic alcohol to diminish the effect of subsequent acute alcohol suggesting development of tolerance. Our results demonstrate that analysis of learning performance of zebrafish allows detection of alcohol-induced functional changes. The simplicity and scalability of the employed task also imply the utility of the zebrafish in high throughput drug screens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping