PUBLICATION
Multistepped optogenetics connects neurons and behavior
- Authors
- Itoh, M., Yamamoto, T., Nakajima, Y., Hatta, K.
- ID
- ZDB-PUB-141217-3
- Date
- 2014
- Source
- Current biology : CB 24: R1155-R1156 (Journal)
- Registered Authors
- Hatta, Kohei
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/embryology
- Animals, Genetically Modified/genetics
- Gene Expression/radiation effects*
- Hot Temperature
- Lasers
- Neurons/cytology
- Neurons/metabolism
- Optogenetics/methods*
- Rhodopsin/genetics*
- Rhodopsin/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 25514003 Full text @ Curr. Biol.
Citation
Itoh, M., Yamamoto, T., Nakajima, Y., Hatta, K. (2014) Multistepped optogenetics connects neurons and behavior. Current biology : CB. 24:R1155-R1156.
Abstract
Our knowledge of the morphology, development and behavioral functions of neurons is limited and yet is essential for understanding the animal nervous system. Optogenetics is a powerful recently developed tool with which to induce behaviors by activating specific neurons [1,2]; however, its successful application often depends on the availability of specific regulatory sequences. Here we describe a multistep optogenetic approach in which an infrared laser is used to induce expression in a small group of neurons in zebrafish of channelorhodopsin (ChR2) fused to a fluorescent protein, and then the morphologies of the neurons and the behaviors induced by blue-light irradiation are investigated (Figure 1A). This strategy can be used to simultaneously explore the anatomy and behavioral functions of known and unknown neurons in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping