ZFIN ID: ZDB-PUB-141217-3
Multistepped optogenetics connects neurons and behavior
Itoh, M., Yamamoto, T., Nakajima, Y., Hatta, K.
Date: 2014
Source: Current biology : CB   24: R1155-R1156 (Journal)
Registered Authors: Hatta, Kohei
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics
  • Gene Expression/radiation effects*
  • Hot Temperature
  • Lasers
  • Neurons/cytology
  • Neurons/metabolism
  • Optogenetics/methods*
  • Rhodopsin/genetics*
  • Rhodopsin/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 25514003 Full text @ Curr. Biol.
Our knowledge of the morphology, development and behavioral functions of neurons is limited and yet is essential for understanding the animal nervous system. Optogenetics is a powerful recently developed tool with which to induce behaviors by activating specific neurons [1,2]; however, its successful application often depends on the availability of specific regulatory sequences. Here we describe a multistep optogenetic approach in which an infrared laser is used to induce expression in a small group of neurons in zebrafish of channelorhodopsin (ChR2) fused to a fluorescent protein, and then the morphologies of the neurons and the behaviors induced by blue-light irradiation are investigated (Figure 1A). This strategy can be used to simultaneously explore the anatomy and behavioral functions of known and unknown neurons in vivo.