|ZFIN ID: ZDB-PUB-141217-3|
Multistepped optogenetics connects neurons and behavior
Itoh, M., Yamamoto, T., Nakajima, Y., Hatta, K.
|Source:||Current biology : CB 24: R1155-R1156 (Journal)|
|Registered Authors:||Hatta, Kohei|
|PubMed:||25514003 Full text @ Curr. Biol.|
Itoh, M., Yamamoto, T., Nakajima, Y., Hatta, K. (2014) Multistepped optogenetics connects neurons and behavior. Current biology : CB. 24:R1155-R1156.
ABSTRACTOur knowledge of the morphology, development and behavioral functions of neurons is limited and yet is essential for understanding the animal nervous system. Optogenetics is a powerful recently developed tool with which to induce behaviors by activating specific neurons [1,2]; however, its successful application often depends on the availability of specific regulatory sequences. Here we describe a multistep optogenetic approach in which an infrared laser is used to induce expression in a small group of neurons in zebrafish of channelorhodopsin (ChR2) fused to a fluorescent protein, and then the morphologies of the neurons and the behaviors induced by blue-light irradiation are investigated (Figure 1A). This strategy can be used to simultaneously explore the anatomy and behavioral functions of known and unknown neurons in vivo.