PUBLICATION
Rapid analysis of hypolipidemic drugs in a live zebrafish assay
- Authors
- Zhou, J., Xu, Y.Q., Guo, S.Y., Li, C.Q.
- ID
- ZDB-PUB-141217-24
- Date
- 2015
- Source
- Journal of Pharmacological and Toxicological Methods 72: 47-52 (Journal)
- Registered Authors
- Keywords
- Bezafibrate (PubChem CID: 39042), Ezetimibe (PubChem CID: 150311), High-fat diet, Hyodesoxycholic acid (PubChem CID: 5283820), Hyperlipidemia, Hypolipidemic drugs, Lipid, Lovastatin (PubChem CID: 53232), Oil Red O staining, Simvastatin (PubChem CID: 54454), Zebrafish
- MeSH Terms
-
- Animals
- Azo Compounds
- Beverages
- Biological Assay/methods*
- Coloring Agents
- Dose-Response Relationship, Drug
- Hypolipidemic Agents/pharmacology*
- Larva/drug effects
- Medicine, Chinese Traditional
- Staining and Labeling
- Time
- Zebrafish
- PubMed
- 25497901 Full text @ J. Pharmacol. Toxicol. Methods
Citation
Zhou, J., Xu, Y.Q., Guo, S.Y., Li, C.Q. (2015) Rapid analysis of hypolipidemic drugs in a live zebrafish assay. Journal of Pharmacological and Toxicological Methods. 72:47-52.
Abstract
Introduction Hyperlipidemia is the most common form of dyslipidemia which is the key risk factor for cardiovascular disease and stroke. The development of effective and safe drug treatments for hyperlipidemia has been proven challenging.
Methods In this study, taking advantage of the transparency of larval zebrafish, we developed a zebrafish hyperlipidemia model for drug screening and efficacy assessment. Zebrafish at 5 d.p.f (days post fertilization) were fed with 0.1% egg yolk for 48h (hours), followed by drug treatment for 24h or 48h. Tested drugs were administered into the zebrafish by direct soaking. Drug effect was evaluated based on quantitative analysis of Oil Red O (ORO) in zebrafish vena caudalis.
Results All 5 human hypolipidemic drugs (simvastatin, lovastatin, ezetimibe, bezafibrate, hyodesoxycholic acid) showed significant hypolipidemic effects (p<0.01) in a dose-dependent manner in the zebrafish hyperlipidemia model. We also found a well-known Chinese tea Pu-erh tea significantly reduced lipids in this model (p<0.001 and p<0.01).
Discussion Our results demonstrate that the zebrafish hyperlipidemia model developed and validated in this study could be used for in vivo hyperlipidemia studies and drug screening and for assessing hypolipidemic drugs with different mechanisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping