PUBLICATION
Expression and functions of ASIC1 in the zebrafish retina
- Authors
- Liu, S., Wang, M.X., Mao, C.J., Cheng, X.Y., Wang, C.T., Huang, J., Zhong, Z.M., Hu, W.D., Wang, F., Hu, L.F., Wang, H., Liu, C.F.
- ID
- ZDB-PUB-141203-62
- Date
- 2014
- Source
- Biochemical and Biophysical Research Communications 455(3-4): 353-7 (Journal)
- Registered Authors
- Wang, Han, Zhong, Zhaomin
- Keywords
- Acid sensing ionic channels, Retina, Zebrafish
- MeSH Terms
-
- Acid Sensing Ion Channels/genetics
- Acid Sensing Ion Channels/physiology*
- Animals
- Gene Expression Regulation, Developmental
- Immunohistochemistry
- In Situ Hybridization
- Larva
- Light
- Microscopy, Fluorescence
- Motor Activity
- Patch-Clamp Techniques
- RNA, Messenger/metabolism
- Retina/metabolism*
- Retinal Ganglion Cells/cytology
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 25446097 Full text @ Biochem. Biophys. Res. Commun.
Citation
Liu, S., Wang, M.X., Mao, C.J., Cheng, X.Y., Wang, C.T., Huang, J., Zhong, Z.M., Hu, W.D., Wang, F., Hu, L.F., Wang, H., Liu, C.F. (2014) Expression and functions of ASIC1 in the zebrafish retina. Biochemical and Biophysical Research Communications. 455(3-4):353-7.
Abstract
It has been demonstrated that acid sensing ionic channels (ASICs) are present in the central and peripheral nervous system of mammals, including the retina. However, it remains unclear whether the zebrafish retina also expresses ASICs. In the present study, the expression and distribution of zasic1 were examined in the retina of zebrafish. Both zasic1 mRNA and protein expressions were detected in the adult zebrafish retina. A wide distribution of ASIC1 in zebrafish retina was confirmed using whole mount in situ hybridization and immunohistochemistry study. Acidosis-induced currents in the isolated retinal ganglion cells (RGCs) were also recorded using whole cell patch clamping. Moreover, blockade of ASICs channel significantly reduced the locomotion of larval zebrafish in response to light exposure. In sum, our data demonstrate the presence of ASIC1 and its possible functional relevance in the retina of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping