PUBLICATION
Targeted analytical toxicology: Simultaneous determination of 17α-ethynylestradiol and the estrogen-induced vitellogenin biomarker
- Authors
- Yang, F., Huang, W., Xie, W., Lu, C., Liu, W.
- ID
- ZDB-PUB-141203-38
- Date
- 2015
- Source
- Environment International 74C: 119-124 (Journal)
- Registered Authors
- Huang, Wei
- Keywords
- 17α-Ethynylestradiol, Environmental toxicology, Protein biomarker, Simultaneous determination, Targeted analytical toxicology, Vitellogenin
- MeSH Terms
-
- Animals
- Biomarkers/analysis
- Biomarkers/metabolism
- Chromatography, Liquid/methods
- Ecotoxicology/methods
- Environmental Pollutants/analysis*
- Environmental Pollutants/metabolism
- Estrogens/analysis*
- Ethinyl Estradiol/analysis*
- Male
- Proteomics/methods*
- Reproducibility of Results
- Spectrometry, Mass, Electrospray Ionization/methods
- Tandem Mass Spectrometry/methods*
- Vitellogenins/analysis*
- Vitellogenins/biosynthesis
- Zebrafish/metabolism
- PubMed
- 25454228 Full text @ Environ. Int.
Citation
Yang, F., Huang, W., Xie, W., Lu, C., Liu, W. (2015) Targeted analytical toxicology: Simultaneous determination of 17α-ethynylestradiol and the estrogen-induced vitellogenin biomarker. Environment International. 74C:119-124.
Abstract
In most toxicological studies, chemical concentrations and its biological/molecular response (biomarker) levels are often quantified separately. We report a method, named as Targeted Analytical Toxicology (TAT), for the simultaneous determination of levels of chemicals and protein biomarkers in organisms by UPLC/ESI-MS/MS on the basis of targeted proteomics. The TAT method is successfully applied to detect the concentrations of 17α-ethynylestradiol (EE2) and EE2-induced vitellogenin (a biomarker for estrogens) in male zebrafish exposed to EE2. It is expected for TAT to have broad application in toxicology, especially in environmental toxicology, due to its high sensitivity, accuracy, reproducibility, high throughput, and the savings of time, cost and labor.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping