PUBLICATION
PFOS affects posterior swim bladder chamber inflation and swimming performance of zebrafish larvae
- Authors
- Hagenaars, A., Stinckens, E., Vergauwen, L., Bervoets, L., Knapen, D.
- ID
- ZDB-PUB-141203-31
- Date
- 2014
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 157C: 225-235 (Journal)
- Registered Authors
- Hagenaars, An, Knapen, Dries, Vergauwen, Lucia
- Keywords
- Early development, PFOS, Swim bladder, Swimming performance, Zebrafish larvae
- MeSH Terms
-
- Air Sacs/drug effects*
- Alkanesulfonic Acids/toxicity*
- Animals
- Body Size/drug effects
- Fluorocarbons/toxicity*
- Larva/drug effects
- Spinal Curvatures/chemically induced
- Spine/drug effects
- Swimming*
- Time Factors
- Water Pollutants, Chemical/toxicity*
- Zebrafish/physiology*
- PubMed
- 25456237 Full text @ Aquat. Toxicol.
Citation
Hagenaars, A., Stinckens, E., Vergauwen, L., Bervoets, L., Knapen, D. (2014) PFOS affects posterior swim bladder chamber inflation and swimming performance of zebrafish larvae. Aquatic toxicology (Amsterdam, Netherlands). 157C:225-235.
Abstract
Perfluorooctane sulphonate (PFOS) is one of the most commonly detected perfluorinated alkylated substances in the aquatic environment due to its persistence and the degradation of less stable compounds to PFOS. PFOS is known to cause developmental effects in fish. The main effect of PFOS in zebrafish larvae is an uninflated swim bladder. As no previous studies have focused on the effect of PFOS on zebrafish swim bladder inflation, the exact mechanisms leading to this effect are currently unknown. The objective of this study was to determine the exposure windows during early zebrafish development that are sensitive to PFOS exposure and result in impaired swim bladder inflation in order to specify the mechanisms by which this effect might be caused. Seven different time windows of exposure (1-48, 1-72, 1-120, 1-144, 48-144, 72-144, 120-144h post fertilization (hpf)) were tested based on the different developmental stages of the swim bladder. These seven time windows were tested for four concentrations corresponding to the EC-values of 1, 10, 80 and 95% impaired swim bladder inflation (EC1=0.70mgL(-1), EC10=1.14mgL(-1), EC80=3.07mgL(-1) and EC95=4.28mgL(-1)). At 6 days post fertilization, effects on survival, hatching, swim bladder inflation and size, larval length and swimming performance were assessed. For 0.70mgL(-1), no significant effects were found for the tested parameters while 1.14mgL(-1) resulted in a reduction of larval length. For 3.07 and 4.28mgL(-1), the number of larvae affected and the severity of effects caused by PFOS were dependent on the time window of exposure. Exposure for 3 days or more resulted in significant reductions of swim bladder size, larval length and swimming speed with increasing severity of effects when the duration of exposure was longer, suggesting a possible effect of accumulated dose. Larvae that were only exposed early (1-48hpf) or late (120-144hpf) during development showed no effects on the studied endpoints. The results demonstrate that PFOS does not affect the budding phase, and does not cause deflation of already inflated swim bladders. PFOS clearly affects processes that take place during the inflation phase and might also have an effect on the formation of the tissue layers forming the swim bladder.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping