PUBLICATION
15 years of zebrafish chemical screening
- Authors
- Rennekamp, A.J., Peterson, R.T.
- ID
- ZDB-PUB-141203-22
- Date
- 2015
- Source
- Current Opinion in Chemical Biology 24C: 58-70 (Other)
- Registered Authors
- Peterson, Randall
- Keywords
- none
- MeSH Terms
-
- Animals
- Drug Evaluation, Preclinical/methods*
- High-Throughput Screening Assays/methods
- Phenotype
- Small Molecule Libraries/chemistry
- Small Molecule Libraries/pharmacology*
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 25461724 Full text @ Curr. Opin. Chem. Biol.
Citation
Rennekamp, A.J., Peterson, R.T. (2015) 15 years of zebrafish chemical screening. Current Opinion in Chemical Biology. 24C:58-70.
Abstract
In 2000, the first chemical screen using living zebrafish in a multi-well plate was reported. Since then, more than 60 additional screens have been published describing whole-organism drug and pathway discovery projects in zebrafish. To investigate the scope of the work reported in the last 14 years and to identify trends in the field, we analyzed the discovery strategies of 64 primary research articles from the literature. We found that zebrafish screens have expanded beyond the use of developmental phenotypes to include behavioral, cardiac, metabolic, proliferative and regenerative endpoints. Additionally, many creative strategies have been used to uncover the mechanisms of action of new small molecules including chemical phenocopy, genetic phenocopy, mutant rescue, and spatial localization strategies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping