PUBLICATION
In vivo imaging of mitochondria in intact zebrafish larvae
- Authors
- Paquet, D., PluciDska, G., Misgeld, T.
- ID
- ZDB-PUB-141125-7
- Date
- 2014
- Source
- Methods in enzymology 547: 151-64 (Chapter)
- Registered Authors
- Paquet, Dominik
- Keywords
- Axonal transport, In vivo imaging, Microscopy, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axonal Transport
- Axons
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism
- Electronic Data Processing
- Embryo, Nonmammalian/cytology
- Image Processing, Computer-Assisted
- Larva/cytology*
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Microscopy, Fluorescence/instrumentation
- Microscopy, Fluorescence/methods*
- Mitochondria/physiology*
- Sensory Receptor Cells/cytology*
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 25416357 Full text @ Methods Enzymol.
Citation
Paquet, D., PluciDska, G., Misgeld, T. (2014) In vivo imaging of mitochondria in intact zebrafish larvae. Methods in enzymology. 547:151-64.
Abstract
Visualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, which allows quantitatively measuring mitochondrial shape, dynamics, and transport in vivo. This provides a useful assay for basic studies exploring the behavior of neuronal mitochondria in their natural habitat, for revealing the influence that disease-related alterations have on this behavior and for testing pharmacological compounds and genetic manipulations that might ameliorate disease-related mitochondrial phenotypes in neurons.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping