PUBLICATION
Ethanol reduces neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos.
- Authors
- Yin, G., Yao, F., Chen, X.H., Wang, N., Wang, H.L., Chang, H.E., Yuan, Z.H., Wu, B.Y.
- ID
- ZDB-PUB-141123-6
- Date
- 2014
- Source
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University 34(11): 1555-61 (Journal)
- Registered Authors
- Chen, Xiaohui
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain
- Cell Differentiation/drug effects*
- Embryo, Nonmammalian/drug effects
- Ethanol/adverse effects*
- Neural Stem Cells/drug effects*
- Neurogenesis/drug effects
- Neuroglia/drug effects*
- Neurons/drug effects*
- Spinal Cord
- Zebrafish*/embryology
- PubMed
- 25413049
Citation
Yin, G., Yao, F., Chen, X.H., Wang, N., Wang, H.L., Chang, H.E., Yuan, Z.H., Wu, B.Y. (2014) Ethanol reduces neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos.. Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 34(11):1555-61.
Abstract
Objective To investigate the influence of exposure to different concentrations of ethanol on neural progenitor cells and the differentiation of neurons and glial cells in zebrafish embryos.
Methods Zebrafish embryos were exposed to 1%, 2%, and 2.5% (V/V) ethanol at 5 hpf by adding ethanol to the egg water. In situ hybridization and real-time PCR were used to detect the changes in the mRNA expression profiles of the markers of different cells to examine the effects of alcohol on neural development.
Results The number of neural precursor cells, neurons and mature glial cells was significantly reduced in the zebrafish embryos following ethanol exposure, and this reduction became more prominent as the ethanol concentration increased. The expression of the early glial marker slc1a3a was down-regulated in the spinal cord but increased in the brain after exposure to increased ethanol concentrations. The expression of the mature glial markers was significantly lowered in response to exposure to increasing ethanol concentrations.
Conclusions Ethanol can reduce neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping