PUBLICATION
High-throughput mapping of brain-wide activity in awake and drug-responsive vertebrates
- Authors
- Lin, X., Wang, S., Yu, X., Liu, Z., Wang, F., Li, W.T., Cheng, S.H., Dai, Q., Shi, P.
- ID
- ZDB-PUB-141120-7
- Date
- 2015
- Source
- Lab on a Chip 15(3): 680-9 (Journal)
- Registered Authors
- Cheng, Shuk Han
- Keywords
- none
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Behavior, Animal/physiology
- Brain/drug effects*
- Brain/physiology*
- Brain Mapping*
- High-Throughput Screening Assays*
- Larva
- Microfluidic Analytical Techniques*
- Neurotoxins/pharmacology
- Peptides/pharmacology
- Wakefulness/physiology*
- Zebrafish
- PubMed
- 25406521 Full text @ Lab Chip
Citation
Lin, X., Wang, S., Yu, X., Liu, Z., Wang, F., Li, W.T., Cheng, S.H., Dai, Q., Shi, P. (2015) High-throughput mapping of brain-wide activity in awake and drug-responsive vertebrates. Lab on a Chip. 15(3):680-9.
Abstract
The reconstruction of neural activity across complete neural circuits, or brain activity mapping, has great potential in both fundamental and translational neuroscience research. Larval zebrafish, a vertebrate model, has recently been demonstrated to be amenable to whole brain activity mapping in behaving animals. Here we demonstrate a microfluidic array system ("Fish-Trap") that enables high-throughput mapping of brain-wide activity in awake larval zebrafish. Unlike the commonly practiced larva-processing methods using a rigid gel or a capillary tube, which are laborious and time-consuming, the hydrodynamic design of our microfluidic chip allows automatic, gel-free, and anesthetic-free processing of tens of larvae for microscopic imaging with single-cell resolution. Notably, this system provides the capability to directly couple pharmaceutical stimuli with real-time recording of neural activity in a large number of animals, and the local and global effects of pharmacoactive drugs on the nervous system can be directly visualized and evaluated by analyzing drug-induced functional perturbation within or across different brain regions. Using this technology, we tested a set of neurotoxin peptides and obtained new insights into how to exploit neurotoxin derivatives as therapeutic agents. The novel and versatile "Fish-Trap" technology can be readily unitized to study other stimulus (optical, acoustic, or physical) associated functional brain circuits using similar experimental strategies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping