ZFIN ID: ZDB-PUB-141030-4
Protective Role of Comfrey Leave Extracts on UV-induced Zebrafish Fin Damage
Cheng, C.C., Chou, C.Y., Chang, Y.C., Wang, H.W., Wen, C.C., Chen, Y.H.
Date: 2014
Source: Journal of Toxicologic Pathology   27: 115-21 (Journal)
Registered Authors: Chen, Yau-Hung
Keywords: UV, comfrey, fin, zebrafish
MeSH Terms: none
PubMed: 25352712 Full text @ J. Toxicol. Pathol.
In zebrafish, UV exposure leads to fin malformation phenotypes including fin reduction or absence. The present study evaluated UV-protective activities of comfrey leaves extracts in a zebrafish model by recording fin morphological changes. Chemopreventive effects of comfrey leave extracts were evaluated using Kaplan-Meier analysis and Cox proportional hazards regression. The results showed that (1) the mean times of return to normal fin in the UV+comfrey (50 and 100 ppm) groups were 3.43 and 2.86 days and were quicker compared with that in the UV only group (4.21 days); (2) zebrafish fins in the UV+comfrey (50 and 100 ppm) groups were 2.05 and 3.25 times more likely to return to normal than those in the UV only group; and (3) comfrey leave extracts had UV-absorbance abilities and significantly reduced ROS production in UV-exposed zebrafish embryos, which may attenuate UV-mediated apoptosis. In conclusion, comfrey leaves extracts may have the potential to be developed as UV-protective agents to protect zebrafish embryos from UV-induced damage.