Identification of a virulence-related surface protein XF in piscine Streptococcus agalactiae by pre-absorbed immunoproteomics
- Authors
- Liu, G., Zhang, W., Liu, Y., Yao, H., Lu, C., Xu, P.
- ID
- ZDB-PUB-141026-1
- Date
- 2014
- Source
- BMC veterinary research 10: 259 (Journal)
- Registered Authors
- Zhang, Wei
- Keywords
- Streptococcus agalactiae, Piscine, Pre-absorbed immunoproteomic method (PAIM), Serine-rich repeat protein (Srr), Zebrafish
- MeSH Terms
-
- Adhesins, Bacterial/genetics
- Adhesins, Bacterial/immunology
- Adhesins, Bacterial/physiology
- Animals
- Fish Diseases/microbiology*
- Hep G2 Cells/microbiology
- Humans
- Immunoblotting/veterinary
- Proteomics
- Real-Time Polymerase Chain Reaction/veterinary
- Streptococcal Infections/microbiology
- Streptococcal Infections/veterinary*
- Streptococcus agalactiae/pathogenicity
- Streptococcus agalactiae/physiology*
- Virulence Factors/genetics
- Virulence Factors/immunology*
- Virulence Factors/physiology
- Zebrafish/microbiology
- PubMed
- 25344337 Full text @ BMC Vet. Res.
Background
Since 2009, large-scale Streptococcus agalactiae infections have broken out in cultured tilapia farms in China, resulting in considerable economic losses. Screening of the surface proteins is required to identify virulence factors or protective antigens involved in piscine S.agalactiae infections in tilapia. Pre-absorbed immunoproteomics method (PAIM) is a useful method previously established in our laboratory for identifying bacterial surface proteins.
Results
A serine-rich repeat protein family 1 (Srr-1), designated XF, was identified by PAIM in piscine S. agalactiae isolate GD201008-001. To investigate the role of XF in the pathogenesis of piscine S. agalactiae, an isogenic xf mutant strain (Δxf) and a complemented strain (CΔxf) were successfully constructed. The Δxf mutant and CΔxf showed no significant differences in growth characteristics and adherence to HEp-2 cells compared with the wild-type strain. However the 50% lethal dose of Δxf was increased (4-fold) compared with that of the parental strain in a zebrafish infection model.
Conclusions
The findings demonstrated that XF is a virulence-related, highly immunoreactive surface protein and is involved in the pathogenicity of S. agalactiae infections in fish.