PUBLICATION

In Vivo Visualization of the Development of the Enteric Nervous System Using a Tg(-8.3bphox2b:Kaede) Transgenic Zebrafish

Authors
Harrison, C., Wabbersen, T., Shepherd, I.
ID
ZDB-PUB-140930-5
Date
2014
Source
Genesis (New York, N.Y. : 2000)   52(12): 985-90 (Journal)
Registered Authors
Shepherd, Iain T.
Keywords
Enteric nervous system, Kaede, in vivo, med24, phox2b
MeSH Terms
  • Animals
  • Animals, Genetically Modified*
  • Enhancer Elements, Genetic
  • Enteric Nervous System/embryology
  • Enteric Nervous System/metabolism*
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Male
  • Organ Specificity
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
25264359 Full text @ Genesis
Abstract
The phox2b gene encodes a transcription factor that is expressed in the developing enteric nervous system (ENS). An enhancer element has been identified in the zebrafish phox2b locus that can drive tissue specific expression of reporter genes in enteric neuron precursor cells. We have generated a transgenic zebrafish line in which the Kaede fluorescent protein is under the control of this phox2b enhancer. This line has stable expression of the Kaede protein in enteric neuron precursor cells over 3 generations. To demonstrate the utility of this line we compared the migration and division rates of enteric neuron precursor cells in wild type and the zebrafish ENS mutant lessen.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping