PUBLICATION
Using correlative light and electron microscopy to study zebrafish vascular morphogenesis
- Authors
- Goetz, J.G., Monduc, F., Schwab, Y., Vermot, J.
- ID
- ZDB-PUB-140924-6
- Date
- 2015
- Source
- Methods in molecular biology (Clifton, N.J.) 1189: 31-46 (Journal)
- Registered Authors
- Vermot, Julien
- Keywords
- none
- MeSH Terms
-
- Anesthesia
- Animals
- Blood Vessels/embryology*
- Blood Vessels/ultrastructure*
- Embryo, Nonmammalian/ultrastructure
- Fluorescence
- Image Processing, Computer-Assisted
- Lasers
- Microscopy, Electron/methods*
- Morphogenesis*
- Resins, Synthetic
- Tissue Fixation
- Tomography
- Zebrafish/embryology*
- PubMed
- 25245685 Full text @ Meth. Mol. Biol.
Citation
Goetz, J.G., Monduc, F., Schwab, Y., Vermot, J. (2015) Using correlative light and electron microscopy to study zebrafish vascular morphogenesis. Methods in molecular biology (Clifton, N.J.). 1189:31-46.
Abstract
Live imaging is extremely useful to characterize the dynamics of cellular events in vivo, yet it is limited in terms of spatial resolution. Correlative light and electron microscopy (CLEM) allows combining live confocal microscopy with electron microscopy (EM) for the characterization of biological samples at high temporal and spatial resolution. Here we describe a protocol allowing extracting endothelial cell ultrastructure after having imaged the same cell in its in vivo context through live confocal imaging during zebrafish embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping