PUBLICATION
Particle-specific toxic effects of differently shaped zno nanoparticles to zebrafish embryos (Danio rerio)
- Authors
- Hua, J., Vijver, M.G., Richardson, M.K., Ahmad, F., Peijnenburg, W.J.
- ID
- ZDB-PUB-140923-1
- Date
- 2014
- Source
- Environmental toxicology and chemistry 33(12): 2859-68 (Journal)
- Registered Authors
- Richardson, Michael
- Keywords
- Mechanism of action, Nanotoxicity, Response addition, Toxic mixtures, Zinc oxide nanoparticles
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects
- Embryo, Nonmammalian/drug effects*
- Ions/chemistry
- Lethal Dose 50
- Metal Nanoparticles/chemistry
- Metal Nanoparticles/toxicity*
- Metal Nanoparticles/ultrastructure
- Microscopy, Electron, Transmission
- Particle Size
- Suspensions/chemistry
- Zebrafish/embryology*
- Zinc Oxide/chemistry*
- Zinc Oxide/toxicity*
- PubMed
- 25244315 Full text @ Environ. Toxicol. Chem.
Citation
Hua, J., Vijver, M.G., Richardson, M.K., Ahmad, F., Peijnenburg, W.J. (2014) Particle-specific toxic effects of differently shaped zno nanoparticles to zebrafish embryos (Danio rerio). Environmental toxicology and chemistry. 33(12):2859-68.
Abstract
A general approach is proposed that allows for quantifying the relative toxic contribution of ions released from metallic nanoparticles and of the particles themselves, as exemplified for the case of differently shaped ZnO nanoparticles exposed to zebrafish embryos. First of all, the toxicity of suspensions of ZnO nanoparticles (NP(total) ): nanospheres, nanosticks, cuboidal submicron particles (SMPs), and Zn(NO3 )2 to the embryos was assessed. The observed toxicity of ZnO NP(total) is assumed to be due to the combined effect of the particles present in the suspensions (NP(particle) ) and of the dissolved Zn(2+) -ions released from the particles (NP(ion) ). Different addition models were used to explicitly account for the toxicity of NP(particle) . The median lethal concentration (LC50 ) of NP(particle) of nanospheres, nanosticks, SMPs were found to range between 7.1 and 11.9 mg Zn/L (i.e. to differ by a factor of 1.7) . Behavioral performance showed no significant differences among all types of the NP(particle) . The median effect concentrations (EC50) of the particles were found to range between 1.0 - 2.2 mg Zn/L). At the LC50 of each particle suspension, the main contribution to lethality to zebrafish embryos was from the NP(particle) (52% - 72%). For hatching inhibition, the NP(particle) was responsible for 38 - 83 % of the adverse effects observed. ZnO nanosticks were more toxic than any of the other NPs with regard to the endpoints mortality and hatching inhibition. The main contribution to toxicity to zebrafish embryos was from the NP(particle) at the L(E)C50 of each particle suspension.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping