PUBLICATION
Retinal Regeneration Following OCT-guided Laser Injury in Zebrafish
- Authors
- DiCicco, R.M., Bell, B.A., Kaul, C., Hollyfield, J.G., Anand-Apte, B., Perkins, B.D., Tao, Y.K., Yuan, A.
- ID
- ZDB-PUB-140911-2
- Date
- 2014
- Source
- Investigative ophthalmology & visual science 55(10): 6281-8 (Journal)
- Registered Authors
- Anand-Apte, Bela, Perkins, Brian
- Keywords
- none
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Follow-Up Studies
- Laser Coagulation/adverse effects
- Ophthalmoscopy
- Retina/injuries
- Retina/pathology*
- Retinal Degeneration/etiology
- Retinal Degeneration/pathology*
- Tomography, Optical Coherence/methods*
- Zebrafish
- PubMed
- 25205862 Full text @ Invest. Ophthalmol. Vis. Sci.
Citation
DiCicco, R.M., Bell, B.A., Kaul, C., Hollyfield, J.G., Anand-Apte, B., Perkins, B.D., Tao, Y.K., Yuan, A. (2014) Retinal Regeneration Following OCT-guided Laser Injury in Zebrafish. Investigative ophthalmology & visual science. 55(10):6281-8.
Abstract
Purpose: Establish a focal injury/regeneration model in zebrafish using laser photocoagulation guided by optical coherence tomography (OCT). Methods: Adult zebrafish were imaged by OCT and confocal scanning laser ophthalmoscopy (cSLO) in room air through a contact lens. Using a beam combiner, 532nm laser photocoagulation was applied using the OCT C-scan image for targeting. Laser spots of 42-47mW were delivered to the retina. At multiple intervals post-injury, fish were imaged using both OCT and cSLO to follow the progression of each lesion. Histologic sections and TUNEL staining were performed to monitor the injury response. Results: Round lesions (26057 ± 621μm2) localized to the outer retina were successfully applied. Laser application was visualized real-time by OCT and lesions were detectable by both OCT and cSLO in vivo. Lesion size increased 1 day post-lesion then decreased in size. Histologic sections showed focal areas of damage localized primarily to the outer retina. By 3 weeks, the damaged areas had regenerated and a fully laminated structure was re-established. However, subtle changes can still be detected by OCT, cSLO imaging, and histology. Infrared darkfield imaging was more sensitive than OCT at revealing subtle changes in regenerated areas. Conclusions: OCT guided laser photocoagulation is a useful tool for inducing localized lesions and studying retinal regeneration in zebrafish. This novel method will allow us to characterize the cellular and molecular changes that take place at the interface between normal and damaged tissue. Regeneration can be observed using high resolution OCT and cSLO imaging in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping