ZFIN ID: ZDB-PUB-140905-7
Perturbation of cytosolic calcium by 2-aminoethoxydiphenyl borate and caffeine affects zebrafish myofibril alignment
Wu, H.J., Fong, T.H., Chen, S.L., Wei, J.C., Wang, I.J., Wen, C.C., Chang, C.Y., Chen, X.G., Chen, W.Y., Chen, H.M., Horng, J.L., Wang, Y.H., Chen, Y.H.
Date: 2015
Source: Journal of applied toxicology : JAT   35(3): 287-94 (Journal)
Registered Authors: Chen, Yau-Hung, Horng, Jiun-Lin, Wang, I-Jong
Keywords: caffeine, calcium, embryogenesis, myogenesis, zebrafish
MeSH Terms:
  • Animals
  • Boron Compounds/toxicity*
  • Caffeine/toxicity*
  • Calcium/metabolism*
  • Cytosol/drug effects*
  • Cytosol/metabolism
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/ultrastructure
  • Microscopy, Electron, Transmission
  • Muscle Development/drug effects*
  • Myofibrils/drug effects*
  • Myofibrils/ultrastructure
  • Zebrafish/embryology*
PubMed: 25186829 Full text @ J. Appl. Toxicol.
ABSTRACT
The objective of the current study was to investigate the effects of Ca(2+) levels on myofibril alignment during zebrafish embryogenesis. To investigate how altered cytoplasmic Ca(2+) levels affect myofibril alignment, we exposed zebrafish embryos to 2-aminothoxyldiphenyl borate (2-APB; an inositol 1,4,5-trisphosphate receptor inhibitor that reduces cytosolic Ca(2+) levels) and caffeine (a ryanodine receptor activator that enhances cytosolic Ca(2+) levels). The results demonstrated that the most evident changes in zebrafish embryos treated with 2-APB were shorter body length, curved trunk and malformed somite boundary. In contrast, such malformed phenotypes were evident neither in untreated controls nor in caffeine-treated embryos. Subtle morphological changes, including changes in muscle fibers, F-actin and ultrastructures were easily observed by staining with specific monoclonal antibodies (F59 and α-laminin), fluorescent probes (phalloidin) and by transmission electron microscopy. Our data suggested that: (1) the exposure to 2-APB and/or caffeine led to myofibril misalignment; (2) 2-APB-treated embryos displayed split and short myofibril phenotypes, whereas muscle fibers from caffeine-treated embryos were twisted and wavy; and (3) zebrafish embryos co-exposed to 2-APB and caffeine resulted in normal myofibril alignment. In conclusion, we proposed that cytosolic Ca(2+) is important for myogenesis, particularly for myofibril alignment.
ADDITIONAL INFORMATION