PUBLICATION
Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays
- Authors
- Perrichon, P., Le Bihanic, F., Bustamante, P., Le Menach, K., Budzinski, H., Cachot, J., Cousin, X.
- ID
- ZDB-PUB-140903-12
- Date
- 2014
- Source
- Environmental science and pollution research international 21(24): 13703-19 (Journal)
- Registered Authors
- Cousin, Xavier
- Keywords
- none
- MeSH Terms
-
- Animals
- Biological Assay/instrumentation
- Biological Assay/methods
- Embryonic Development/drug effects
- Environmental Monitoring/instrumentation
- Environmental Monitoring/methods
- Geologic Sediments/chemistry*
- Larva/drug effects*
- Larva/growth & development
- Oryzias/embryology*
- Polycyclic Aromatic Hydrocarbons/analysis
- Polycyclic Aromatic Hydrocarbons/toxicity*
- Water Pollutants, Chemical/analysis
- Water Pollutants, Chemical/toxicity*
- Zebrafish/growth & development*
- PubMed
- 25175355 Full text @ Environ. Sci. Pollut. Res. Int.
Citation
Perrichon, P., Le Bihanic, F., Bustamante, P., Le Menach, K., Budzinski, H., Cachot, J., Cousin, X. (2014) Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays. Environmental science and pollution research international. 21(24):13703-19.
Abstract
Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10-day zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals testing. Second, a comparative study of toxicity responses from this exposure protocol was then performed using another OECD species, the Japanese medaka. The potential toxicity of both PAHs was assessed through lethal (e.g., survival, hatching success) and sublethal endpoints (e.g., abnormalities, PMR, and EROD) measured at different developmental stages, adapted to the embryonic development time of both species. Regarding effects observed for both species, a suitable artificial sediment composition for PAH toxicity testing was set at 92.5 % dry weight (dw) silica of 0.2-0.5-mm grain size, 5 % dw kaolin clay without organic matter for zebrafish, and 2.5 % dw blond peat in more only for Japanese medaka. PAH bioavailability and toxicity were highly dependent on the fraction of organic matter in sediment and of the K ow coefficients of the tested compounds. The biological responses observed were also dependent of the species under consideration. Japanese medaka embryos appeared more robust than zebrafish embryos for understanding the toxicity of PAHs following a sediment contact test, due to the longer exposure duration and lower sensitivity of sediment physical properties.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping