PUBLICATION
Sequential Gene Silencing Using Wavelength-Selective Caged Morpholino Oligonucleotides
- Authors
- Yamazoe, S., Liu, Q., McQuade, L.E., Deiters, A., Chen, J.K.
- ID
- ZDB-PUB-140819-4
- Date
- 2014
- Source
- Angewandte Chemie (International ed. in English) 53(38): 10114-8 (Journal)
- Registered Authors
- Chen, James K.
- Keywords
- antisense agents, cage compounds, developmental biology, gene expression, oligonucleotides
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Gene Silencing*
- Molecular Structure
- Morpholinos/chemistry
- Morpholinos/genetics*
- Zebrafish/embryology
- PubMed
- 25130695 Full text @ Angew. Chem. Int. Ed. Engl.
Citation
Yamazoe, S., Liu, Q., McQuade, L.E., Deiters, A., Chen, J.K. (2014) Sequential Gene Silencing Using Wavelength-Selective Caged Morpholino Oligonucleotides. Angewandte Chemie (International ed. in English). 53(38):10114-8.
Abstract
Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength-selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse-genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping