ZFIN ID: ZDB-PUB-140819-1
A Self-Organizing miR-132/Ctbp2 Circuit Regulates Bimodal Notch Signals and Glial Progenitor Fate Choice during Spinal Cord Maturation
Salta, E., Lau, P., Sala Frigerio, C., Coolen, M., Bally-Cuif, L., De Strooper, B.
Date: 2014
Source: Developmental Cell   30(4): 423-36 (Journal)
Registered Authors: Bally-Cuif, Laure, Coolen, Marion
Keywords: none
MeSH Terms:
  • Animals
  • Cell Differentiation*
  • Feedback, Physiological
  • Gene Expression Regulation, Developmental
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Microglia/cytology
  • Microglia/metabolism*
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism*
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism*
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism*
  • Sirtuin 1/genetics
  • Sirtuin 1/metabolism
  • Spinal Cord/cytology
  • Spinal Cord/embryology
  • Spinal Cord/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 25132384 Full text @ Dev. Cell
Radial glial progenitors play pivotal roles in the development and patterning of the spinal cord, and their fate is controlled by Notch signaling. How Notch is shaped to regulate their crucial transition from expansion toward differentiation remains, however, unknown. miR-132 in the developing zebrafish dampens Notch signaling via a cascade involving the transcriptional corepressor Ctbp2 and the Notch suppressor Sirt1. At early embryonic stages, high Ctbp2 levels sustain Notch signaling and radial glial expansion and concomitantly induce miR-132 expression via a double-negative feedback loop involving Rest inhibition. The changing balance in miR-132 and Ctbp2 interaction gradually drives the switch in Notch output and radial glial progenitor fate as part of the larger developmental program involved in the transition from embryonic to larval spinal cord.