PUBLICATION

Understanding cardiac sarcomere assembly with zebrafish genetics

Authors
Yang, J., Shih, Y.H., Xu, X.
ID
ZDB-PUB-140816-8
Date
2014
Source
Anatomical record (Hoboken, N.J. : 2007)   297: 1681-93 (Review)
Registered Authors
Shih, Yu-huan, Xu, Xiaolei, Yang, Jingchun
Keywords
cardiomyopathy, genetics, myofibrillogenesis, sarcomere, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Genetic Predisposition to Disease
  • Genotype
  • Heart Diseases/genetics
  • Heart Diseases/metabolism
  • Heart Diseases/pathology
  • Humans
  • Mutation
  • Myocytes, Cardiac/metabolism*
  • Myocytes, Cardiac/pathology
  • Phenotype
  • Risk Factors
  • Sarcomeres/metabolism*
  • Sarcomeres/pathology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
25125181 Full text @ Anat. Rec. (Hoboken)
Abstract
Mutations in sarcomere genes have been found in many inheritable human diseases, including hypertrophic cardiomyopathy. Elucidating the molecular mechanisms of sarcomere assembly shall facilitate understanding of the pathogenesis of sarcomere-based cardiac disease. Recently, biochemical and genomic studies have identified many new genes encoding proteins that localize to the sarcomere. However, their precise functions in sarcomere assembly and sarcomere-based cardiac disease are unknown. Here, we review zebrafish as an emerging vertebrate model for these studies. We summarize the techniques offered by this animal model to manipulate genes of interest, annotate gene expression, and describe the resulting phenotypes. We survey the sarcomere genes that have been investigated in zebrafish and discuss the potential of applying this in vivo model for larger-scale genetic studies.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping