PUBLICATION
The biofilm mode of life boosts the anti-inflammatory properties of Lactobacillus
- Authors
- Rieu, A., Aoudia, N., Jego, G., Chluba, J., Yousfi, N., Briandet, R., Deschamps, J., Gasquet, B., Monedero, V., Garrido, C., Guzzo, J.
- ID
- ZDB-PUB-140724-7
- Date
- 2014
- Source
- Cellular Microbiology 16(12): 1836-53 (Journal)
- Registered Authors
- Yousfi, Nadhir
- Keywords
- none
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/metabolism
- Bacterial Adhesion*
- Biofilms/growth & development*
- Cell Line
- Chaperonin 60/metabolism
- Gastrointestinal Tract/microbiology
- Humans
- Immune Tolerance
- Lacticaseibacillus casei/immunology*
- Lacticaseibacillus casei/metabolism
- Lacticaseibacillus casei/physiology*
- Larva/microbiology
- Macrophages/immunology
- Tumor Necrosis Factor-alpha
- Zebrafish/immunology*
- Zebrafish/microbiology*
- PubMed
- 25052472 Full text @ Cell. Microbiol.
Citation
Rieu, A., Aoudia, N., Jego, G., Chluba, J., Yousfi, N., Briandet, R., Deschamps, J., Gasquet, B., Monedero, V., Garrido, C., Guzzo, J. (2014) The biofilm mode of life boosts the anti-inflammatory properties of Lactobacillus. Cellular Microbiology. 16(12):1836-53.
Abstract
The predominant form of life for microorganisms in their natural habitats is the biofilm mode of growth. The adherence and colonization of probiotic bacteria are considered as essential factors for their immunoregulatory function in the host. Here, we show that Lactobacillus casei ATCC334 adheres to and colonizes the gut of zebrafish larvae. The abundance of pro-inflammatory cytokines and the recruitment of macrophages were low when inflammation was induced in probiotic-fed animals, suggesting that these bacteria have anti-inflammatory properties. We treated human macrophage-differentiated monocytic THP-1 cells with supernatants of L. casei ATCC334 grown in either biofilm or planktonic cultures. TNF-α production was suppressed and the NF-κB pathway was inhibited only in the presence of supernatants from biofilms. We identified GroEL as the biofilm supernatant compound responsible, at least partially, for this anti-inflammatory effect. Gradual immunodepletion of GroEL demonstrated that the abundance of GroEL and TNF-α were inversely correlated. We confirmed that biofilm development in other Lactobacillus species affects the immune response. The biofilms supernatants of these species also contained large amounts of GroEL. Thus, our results demonstrate that the biofilm enhances the immunomodulatory effects of Lactobacillus sp. and that secreted GroEL is involved in this beneficial effect.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping