PUBLICATION

Type-IV Antifreeze Proteins are Essential for Epiboly and Convergence in Gastrulation of Zebrafish Embryos

Authors
Xiao, Q., Xia, J.H., Zhang, X.J., Li, Z., Wang, Y., Zhou, L., Gui, J.F.
ID
ZDB-PUB-140712-4
Date
2014
Source
International journal of biological sciences   10: 715-32 (Journal)
Registered Authors
Gui, Jian-Fang, Li, Zhi, Wang, Yang, Zhang, Xiao-juan, Zhou, Li
Keywords
antifreeze protein, convergence, epiboly, morphogenesis., yolk syncytial layer
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Antifreeze Proteins, Type IV/genetics
  • Antifreeze Proteins, Type IV/metabolism
  • Antifreeze Proteins, Type IV/physiology*
  • Body Patterning/genetics
  • Cloning, Molecular
  • Gastrulation/genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed
25013380 Full text @ Int. J. Biol. Sci.
Abstract
Many organisms in extremely cold environments such as the Antarctic Pole have evolved antifreeze molecules to prevent ice formation. There are four types of antifreeze proteins (AFPs). Type-IV antifreeze proteins (AFP4s) are present also in certain temperate and even tropical fish, which has raised a question as to whether these AFP4s have important functions in addition to antifreeze activity. Here we report the identification and functional analyses of AFP4s in cyprinid fish. Two genes, namely afp4a and afp4b coding for AFP4s, were identified in gibel carp (Carassius auratus gibelio) and zebrafish (Danio rerio). In both species, afp4a and afp4b display a head-to-tail tandem arrangement and share a common 4-exonic gene structure. In zebrafish, both afp4a and afp4b were found to express specifically in the yolk syncytial layer (YSL). Interestingly, afp4a expression continues in YSL and digestive system from early embryos to adults, whereas afp4b expression is restricted to embryogenesis. Importantly, we have shown by using afp4a-specific and afp4b-specifc morpholino knockdown and cell lineage tracing approaches that AFP4a participates in epiboly progression by stabilizing yolk cytoplasmic layer microtubules, and AFP4b is primarily related to convergence movement. Therefore, both AFP4 proteins are essential for gastrulation of zebrafish embryos. Our current results provide first evidence that AFP such as AFP4 has important roles in regulating developmental processes besides its well-known function as antifreeze factors.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping