|ZFIN ID: ZDB-PUB-140712-10|
Collective epithelial migration drives kidney repair after acute injury
Palmyre, A., Lee, J., Ryklin, G., Camarata, T., Selig, M.K., Duchemin, A.L., Nowak, P., Arnaout, M.A., Drummond, I.A., Vasilyev, A.
|Source:||PLoS One 9: e101304 (Journal)|
|Registered Authors:||Drummond, Iain, Duchemin, Anne-Laure, Vasilyev, Aleksandr|
|PubMed:||25010471 Full text @ PLoS One|
Palmyre, A., Lee, J., Ryklin, G., Camarata, T., Selig, M.K., Duchemin, A.L., Nowak, P., Arnaout, M.A., Drummond, I.A., Vasilyev, A. (2014) Collective epithelial migration drives kidney repair after acute injury. PLoS One. 9:e101304.
ABSTRACTAcute kidney injury (AKI) is a common and significant medical problem. Despite the kidney's remarkable regenerative capacity, the mortality rate for the AKI patients is high. Thus, there remains a need to better understand the cellular mechanisms of nephron repair in order to develop new strategies that would enhance the intrinsic ability of kidney tissue to regenerate. Here, using a novel, laser ablation-based, zebrafish model of AKI, we show that collective migration of kidney epithelial cells is a primary early response to acute injury. We also show that cell proliferation is a late response of regenerating kidney epithelia that follows cell migration during kidney repair. We propose a computational model that predicts this temporal relationship and suggests that cell stretch is a mechanical link between migration and proliferation, and present experimental evidence in support of this hypothesis. Overall, this study advances our understanding of kidney repair mechanisms by highlighting a primary role for collective cell migration, laying a foundation for new approaches to treatment of AKI.