PUBLICATION
Collective epithelial migration drives kidney repair after acute injury
- Authors
- Palmyre, A., Lee, J., Ryklin, G., Camarata, T., Selig, M.K., Duchemin, A.L., Nowak, P., Arnaout, M.A., Drummond, I.A., Vasilyev, A.
- ID
- ZDB-PUB-140712-10
- Date
- 2014
- Source
- PLoS One 9: e101304 (Journal)
- Registered Authors
- Drummond, Iain, Duchemin, Anne-Laure, Vasilyev, Aleksandr
- Keywords
- none
- MeSH Terms
-
- Acute Kidney Injury/pathology*
- Acute Kidney Injury/physiopathology*
- Animals
- Cell Movement*
- Cell Proliferation
- Epithelial Cells/pathology*
- Epithelial-Mesenchymal Transition
- Kidney/pathology*
- Kidney/physiopathology*
- Lasers
- Models, Biological
- Zebrafish
- PubMed
- 25010471 Full text @ PLoS One
Citation
Palmyre, A., Lee, J., Ryklin, G., Camarata, T., Selig, M.K., Duchemin, A.L., Nowak, P., Arnaout, M.A., Drummond, I.A., Vasilyev, A. (2014) Collective epithelial migration drives kidney repair after acute injury. PLoS One. 9:e101304.
Abstract
Acute kidney injury (AKI) is a common and significant medical problem. Despite the kidney's remarkable regenerative capacity, the mortality rate for the AKI patients is high. Thus, there remains a need to better understand the cellular mechanisms of nephron repair in order to develop new strategies that would enhance the intrinsic ability of kidney tissue to regenerate. Here, using a novel, laser ablation-based, zebrafish model of AKI, we show that collective migration of kidney epithelial cells is a primary early response to acute injury. We also show that cell proliferation is a late response of regenerating kidney epithelia that follows cell migration during kidney repair. We propose a computational model that predicts this temporal relationship and suggests that cell stretch is a mechanical link between migration and proliferation, and present experimental evidence in support of this hypothesis. Overall, this study advances our understanding of kidney repair mechanisms by highlighting a primary role for collective cell migration, laying a foundation for new approaches to treatment of AKI.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping