PUBLICATION

Collective epithelial migration drives kidney repair after acute injury

Authors
Palmyre, A., Lee, J., Ryklin, G., Camarata, T., Selig, M.K., Duchemin, A.L., Nowak, P., Arnaout, M.A., Drummond, I.A., Vasilyev, A.
ID
ZDB-PUB-140712-10
Date
2014
Source
PLoS One   9: e101304 (Journal)
Registered Authors
Drummond, Iain, Duchemin, Anne-Laure, Vasilyev, Aleksandr
Keywords
none
MeSH Terms
  • Acute Kidney Injury/pathology*
  • Acute Kidney Injury/physiopathology*
  • Animals
  • Cell Movement*
  • Cell Proliferation
  • Epithelial Cells/pathology*
  • Epithelial-Mesenchymal Transition
  • Kidney/pathology*
  • Kidney/physiopathology*
  • Lasers
  • Models, Biological
  • Zebrafish
PubMed
25010471 Full text @ PLoS One
Abstract
Acute kidney injury (AKI) is a common and significant medical problem. Despite the kidney's remarkable regenerative capacity, the mortality rate for the AKI patients is high. Thus, there remains a need to better understand the cellular mechanisms of nephron repair in order to develop new strategies that would enhance the intrinsic ability of kidney tissue to regenerate. Here, using a novel, laser ablation-based, zebrafish model of AKI, we show that collective migration of kidney epithelial cells is a primary early response to acute injury. We also show that cell proliferation is a late response of regenerating kidney epithelia that follows cell migration during kidney repair. We propose a computational model that predicts this temporal relationship and suggests that cell stretch is a mechanical link between migration and proliferation, and present experimental evidence in support of this hypothesis. Overall, this study advances our understanding of kidney repair mechanisms by highlighting a primary role for collective cell migration, laying a foundation for new approaches to treatment of AKI.
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