PUBLICATION
Ancient Origins of RGK Protein Function: Modulation of Voltage-Gated Calcium Channels Preceded the Protostome and Deuterostome Split
- Authors
- Puhl, H.L., Lu, V.B., Won, Y.J., Sasson, Y., Hirsch, J.A., Ono, F., Ikeda, S.R.
- ID
- ZDB-PUB-140706-12
- Date
- 2014
- Source
- PLoS One 9: e100694 (Journal)
- Registered Authors
- Ono, Fumihito
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Calcium Channels, L-Type/genetics*
- Calcium Channels, L-Type/metabolism
- Drosophila Proteins/genetics*
- Drosophila Proteins/metabolism
- Drosophila melanogaster
- Evolution, Molecular*
- Molecular Sequence Data
- Monomeric GTP-Binding Proteins/genetics*
- Monomeric GTP-Binding Proteins/metabolism
- Rats
- Rats, Wistar
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- ras Proteins/genetics*
- ras Proteins/metabolism
- PubMed
- 24992013 Full text @ PLoS One
Citation
Puhl, H.L., Lu, V.B., Won, Y.J., Sasson, Y., Hirsch, J.A., Ono, F., Ikeda, S.R. (2014) Ancient Origins of RGK Protein Function: Modulation of Voltage-Gated Calcium Channels Preceded the Protostome and Deuterostome Split. PLoS One. 9:e100694.
Abstract
RGK proteins, Gem, Rad, Rem1, and Rem2, are members of the Ras superfamily of small GTP-binding proteins that interact with Ca2+ channel β subunits to modify voltage-gated Ca2+ channel function. In addition, RGK proteins affect several cellular processes such as cytoskeletal rearrangement, neuronal dendritic complexity, and synapse formation. To probe the phylogenetic origins of RGK protein-Ca2+ channel interactions, we identified potential RGK-like protein homologs in genomes for genetically diverse organisms from both the deuterostome and protostome animal superphyla. RGK-like protein homologs cloned from Danio rerio (zebrafish) and Drosophila melanogaster (fruit flies) expressed in mammalian sympathetic neurons decreased Ca2+ current density as reported for expression of mammalian RGK proteins. Sequence alignments from evolutionarily diverse organisms spanning the protostome/deuterostome divide revealed conservation of residues within the RGK G-domain involved in RGK protein - Cavβ subunit interaction. In addition, the C-terminal eleven residues were highly conserved and constituted a signature sequence unique to RGK proteins but of unknown function. Taken together, these data suggest that RGK proteins, and the ability to modify Ca2+ channel function, arose from an ancestor predating the protostomes split from deuterostomes approximately 550 million years ago.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping