PUBLICATION
The Serine-Threonine Protein Kinase PAK4 Is Dispensable in Zebrafish: Identification of a Morpholino-Generated Pseudophenotype
- Authors
- Law, S.H., Sargent, T.D.
- ID
- ZDB-PUB-140620-5
- Date
- 2014
- Source
- PLoS One 9: e100268 (Journal)
- Registered Authors
- Sargent, Tom
- Keywords
- none
- MeSH Terms
-
- Alleles
- Animals
- Base Sequence
- Endonucleases/metabolism
- Female
- Gene Knockout Techniques
- INDEL Mutation/genetics
- Male
- Molecular Sequence Data
- Morpholinos/pharmacology*
- Mutation/genetics
- Phenotype
- RNA/genetics
- RNA/metabolism
- Stress, Physiological/drug effects
- Stress, Physiological/genetics
- Trans-Activators/metabolism
- Zebrafish/embryology
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- p21-Activated Kinases/genetics
- p21-Activated Kinases/metabolism*
- PubMed
- 24945275 Full text @ PLoS One
Citation
Law, S.H., Sargent, T.D. (2014) The Serine-Threonine Protein Kinase PAK4 Is Dispensable in Zebrafish: Identification of a Morpholino-Generated Pseudophenotype. PLoS One. 9:e100268.
Abstract
TALEN-based inactivation of the zebrafish pak4 gene resulted in embryos and adult fish that appear normal and fertile. This is in contrast to our previously published studies which were based on the use of antisense morpholino oligonucleotides (MOs). We have excluded potential explanations such as gene duplication, alternate splicing, cryptic initiation of translation, and translation-independent RNA function. Our conclusion is that pak4 is dispensable in zebrafish, and that even when corroborated by robust controls, such as RNA rescue, MOs may elicit misleading pseudophenotypes that do not correspond to results obtained by genetic mutations, and should thus be used with caution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping