ZFIN ID: ZDB-PUB-140619-6
Hydrogen sulfide inhibits Na(+) uptake in larval zebrafish, Danio rerio
Kumai, Y., Porteus, C.S., Kwong, R.W., Perry, S.F.
Date: 2015
Source: Pflugers Archiv : European journal of physiology   467(4): 651-64 (Journal)
Registered Authors: Perry, Steve F.
Keywords: none
MeSH Terms:
  • Animals
  • Cystathionine beta-Synthase/antagonists & inhibitors
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Epithelial Cells/drug effects
  • Epithelial Cells/metabolism
  • Hydrogen Sulfide/pharmacology*
  • Larva/drug effects
  • Larva/metabolism
  • Lyases/antagonists & inhibitors
  • Lyases/genetics
  • Lyases/metabolism
  • Skin Absorption*
  • Sodium/metabolism*
  • Sodium Chloride Symporters/metabolism
  • Sodium-Hydrogen Exchangers/genetics
  • Sodium-Hydrogen Exchangers/metabolism
  • Sulfites/pharmacology
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 24939700 Full text @ Pflügers Archiv. / Eur. J. Physiol.
ABSTRACT
The present study investigated the role of hydrogen sulfide (H2S) in regulating Na(+) uptake in larval zebrafish, Danio rerio. Waterborne treatment of larvae at 4 days post-fertilization (dpf) with Na2S or GYY-4137 (chemicals known to generate H2S) significantly reduced Na(+) uptake. Exposure of larvae to water enriched with NaCl (1 mM NaCl) caused a pronounced reduction in Na(+) uptake which was prevented by pharmacological inhibition of cystathionine β-synthase (CBS) or cystathionine γ-lyase (CSE), two key enzymes involved in the endogenous synthesis of H2S. Furthermore, translational gene knockdown of CSE and CBSb significantly increased the basal rate of Na(+) uptake. Waterborne treatment with Na2S significantly decreased whole-body acid excretion and reduced Na(+) uptake in larval zebrafish preexposed to acidic (pH 4.0) water (a condition shown to promote Na(+) uptake via Na(+)-H(+)-exchanger 3b, NHE3b). However, Na2S did not affect Na(+) uptake in larvae depleted of NHE3b-containing ionocytes (HR cells) after knockdown of transcription factor glial cell missing 2 (gcm2) in which Na(+) uptake occurs predominantly via Na(+)-Cl(-) co-transporter (NCC)-containing cells. These observations suggest that Na(+) uptake via NHE3b, but not NCC, is regulated by H2S. Whole-mount immunohistochemistry demonstrated that ionocytes expressing NHE3b also express CSE. These data suggests a physiologically relevant role of H2S as a mechanism to lower Na(+) uptake in zebrafish larvae, probably through its inhibitory action on NHE3b.
ADDITIONAL INFORMATION