PUBLICATION
Acute Net Stressor Increases Whole-Body Cortisol Levels Without Altering Whole-Brain Monoamines in Zebrafish
- Authors
- Tran, S., Chatterjee, D., Gerlai, R.
- ID
- ZDB-PUB-140610-4
- Date
- 2014
- Source
- Behavioral neuroscience 128(5): 621-4 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- none
- MeSH Terms
-
- 3,4-Dihydroxyphenylacetic Acid/metabolism
- Analysis of Variance
- Animals
- Biogenic Monoamines/metabolism*
- Brain/metabolism*
- Dopamine/metabolism
- Enzyme-Linked Immunosorbent Assay
- Female
- Handling, Psychological*
- Hydrocortisone/metabolism*
- Hydroxyindoleacetic Acid/metabolism
- Male
- Serotonin/metabolism
- Stress, Psychological/metabolism*
- Time Factors
- Zebrafish
- PubMed
- 24911317 Full text @ Behav. Neurosci.
Citation
Tran, S., Chatterjee, D., Gerlai, R. (2014) Acute Net Stressor Increases Whole-Body Cortisol Levels Without Altering Whole-Brain Monoamines in Zebrafish. Behavioral neuroscience. 128(5):621-4.
Abstract
Recent studies have started to examine the neurochemical and hormonal basis of behavior in zebrafish by examining biological correlates postmortem. However, it is unknown whether stress involved with experimental handling prior to euthanizing animals will have an impact on subsequent biological measures. In the current study, we expose zebrafish to a short net handling stressor (30 s) and examine the levels of monoamine neurotransmitters (dopamine, serotonin, and their metabolites) and whole-body cortisol at different intervals poststressor (0, 1, 5, 10, and 15 min). We report a time-dependent increase in the levels of cortisol, but no alterations in the levels of dopamine, DOPAC (dopamine's metabolite), serotonin, or 5HIAA (serotonin's metabolite) poststressor. The results demonstrate cortisol levels are more responsive to this type of stimulus compared with neurochemical measures. (PsycINFO Database Record (c) 2014 APA, all rights reserved).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping