PUBLICATION
Amigo Adhesion Protein Regulates Development of Neural Circuits in Zebrafish Brain
- Authors
- Zhao, X., Kuja-Panula, J., Sundvik, M., Chen, Y.C., Aho, V., Peltola, M., Porkka-Heiskanen, T., Panula, P., Rauvala, H.
- ID
- ZDB-PUB-140607-8
- Date
- 2014
- Source
- The Journal of biological chemistry 289(29): 19958-19975 (Journal)
- Registered Authors
- Chen, Yu-Chia, Panula, Pertti, Sundvik, Maria
- Keywords
- Amigo protein family, Axonal scaffold, Kv2.1 potassium channel, Leucine-rich repeat, axon, brain, neurite outgrowth, neurobiology, neurodevelopment, zebrafish
- MeSH Terms
-
- Animals
- Brain/growth & development*
- Brain/metabolism*
- Cell Adhesion Molecules, Neuronal/antagonists & inhibitors
- Cell Adhesion Molecules, Neuronal/genetics
- Cell Adhesion Molecules, Neuronal/metabolism*
- Female
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Larva/growth & development
- Larva/metabolism
- Male
- Nerve Net/growth & development
- Nerve Net/metabolism
- Nerve Tissue Proteins/antagonists & inhibitors
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Neural Cell Adhesion Molecules/antagonists & inhibitors
- Neural Cell Adhesion Molecules/genetics
- Neural Cell Adhesion Molecules/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Shab Potassium Channels/genetics
- Shab Potassium Channels/metabolism
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism*
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 24904058 Full text @ J. Biol. Chem.
Citation
Zhao, X., Kuja-Panula, J., Sundvik, M., Chen, Y.C., Aho, V., Peltola, M., Porkka-Heiskanen, T., Panula, P., Rauvala, H. (2014) Amigo Adhesion Protein Regulates Development of Neural Circuits in Zebrafish Brain. The Journal of biological chemistry. 289(29):19958-19975.
Abstract
The Amigo protein family consists of three transmembrane proteins characterized by six LRR (leucine-rich repeat) domains and one Ig (immunoglobulin-like) domain in their extracellular moieties. Previous in vitro studies have suggested a role as homophilic adhesion molecules in brain neurons but the in vivo functions remain unknown. Here we have cloned all three zebrafish amigos and show that amigo1 is the predominant family member expressed during nervous system development in zebrafish. Knockdown of amigo1 expression using morpholino oligonucleotides impairs the formation of fasciculated tracts in early fiber scaffolds of brain. A similar defect in fiber tract development is caused by mRNA-mediated expression of the Amigo1 ectodomain that inhibits adhesion mediated by the full-length protein. Analysis of differentiated neural circuits reveals defects in the catecholaminergic system. At the behavioral level, the disturbed formation of neural circuitry is reflected in enhanced locomotor activity and in the inability of the larvae to perform normal escape responses. We suggest that Amigo1 is essential for the development of neural circuits of zebrafish where its mechanism involves homophilic interactions within the developing fiber tracts and regulation of the Kv2.1 potassium channel to form functional neural circuitry that controls locomotion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping