ZFIN ID: ZDB-PUB-140601-1
The effects of triclosan on pluripotency factors and development of mouse embryonic stem cells and zebrafish
Chen, X., Xu, B., Han, X., Mao, Z., Chen, M., Du, G., Talbot, P., Wang, X., Xia, Y.
Date: 2015
Source: Archives of toxicology   89(4): 635-46 (Journal)
Registered Authors: Xu, Bo
Keywords: none
MeSH Terms:
  • Animals
  • Apoptosis/drug effects
  • Cell Culture Techniques
  • Cell Cycle/drug effects
  • Cell Survival/drug effects
  • Embryo, Nonmammalian/drug effects*
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/pathology
  • Embryonic Development/drug effects*
  • Endocrine Disruptors/toxicity*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Induced Pluripotent Stem Cells/drug effects*
  • Induced Pluripotent Stem Cells/metabolism
  • Induced Pluripotent Stem Cells/pathology
  • Mice
  • Mouse Embryonic Stem Cells/drug effects*
  • Mouse Embryonic Stem Cells/metabolism
  • Mouse Embryonic Stem Cells/pathology
  • Octamer Transcription Factor-3/genetics
  • Octamer Transcription Factor-3/metabolism
  • SOX Transcription Factors/genetics
  • SOX Transcription Factors/metabolism
  • SOXB1 Transcription Factors/genetics
  • SOXB1 Transcription Factors/metabolism
  • Triclosan/toxicity*
  • Up-Regulation
  • Zebrafish/embryology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 24879426 Full text @ Arch. Toxicol.
ABSTRACT
Triclosan (TCS) poses potential risks to reproduction and development due to its endocrine-disrupting properties. However, the mechanism of TCS's effects on early embryonic development is little known. Embryonic stem cells (ESC) and zebrafish embryos provide valuable models for testing the toxic effects of environmental chemicals on early embryogenesis. In this study, mouse embryonic stem cells (mESC) were acutely exposed to TCS for 24 h, and general cytotoxicity and the effect of TCS on pluripotency were then evaluated. In addition, zebrafish embryos were exposed to TCS from 2- to 24-h post-fertilization (hpf), and their morphology was evaluated. In mESC, alkaline phosphatase staining was significantly decreased after treatment with the highest concentration of TCS (50 μM). Although the expression levels of Sox2 mRNA were not changed, the mRNA levels of Oct4 and Nanog in TCS-treated groups were significantly decreased compared to controls. In addition, the protein levels of Oct4, Sox2 and Nanog were significantly reduced in response to TCS treatment. MicroRNA (miR)-134, an expression inhibitor of pluripotency markers, was significantly increased in TCS-treated mESC. In zebrafish experiments, after 24 hpf of treatment, the controls had developed to the late stage of somitogenesis, while embryos exposed to 300 μg/L of TCS were still at the early stage of somitogenesis, and three genes (Oct4, Sox2 and Nanog) were upregulated in treated groups when compared with the controls. The two models demonstrated that TCS may affect early embryonic development by disturbing the expression of the pluripotency markers (Oct4, Sox2 and Nanog).
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