ZFIN ID: ZDB-PUB-140513-492
Silencing of Odorant Receptor Genes by G Protein βγ Signaling Ensures the Expression of One Odorant Receptor per Olfactory Sensory Neuron
Ferreira, T., Wilson, S.R., Choi, Y.G., Risso, D., Dudoit, S., Speed, T.P., Ngai, J.
Date: 2014
Source: Neuron   81(4): 847-59 (Journal)
Registered Authors: Ngai, John
Keywords: none
Microarrays: GEO:GSE53334
MeSH Terms:
  • Animals
  • Axons/metabolism
  • GTP-Binding Protein beta Subunits/metabolism*
  • GTP-Binding Protein gamma Subunits/metabolism*
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology
  • Olfactory Bulb/metabolism*
  • Olfactory Receptor Neurons/metabolism*
  • Receptors, Odorant/genetics
  • Receptors, Odorant/metabolism*
  • Sensory Receptor Cells/metabolism*
  • Signal Transduction/genetics*
  • Zebrafish/metabolism*
PubMed: 24559675 Full text @ Neuron
Olfactory sensory neurons express just one out of a possible ~ 1,000 odorant receptor genes, reflecting an exquisite mode of gene regulation. In one model, once an odorant receptor is chosen for expression, other receptor genes are suppressed by a negative feedback mechanism, ensuring a stable functional identity of the sensory neuron for the lifetime of the cell. The signal transduction mechanism subserving odorant receptor gene silencing remains obscure, however. Here, we demonstrate in the zebrafish that odorant receptor gene silencing is dependent on receptor activity. Moreover, we show that signaling through G protein βγ subunits is both necessary and sufficient to suppress the expression of odorant receptor genes and likely acts through histone methylation to maintain the silenced odorant receptor genes in transcriptionally inactive heterochromatin. These results link receptor activity with the epigenetic mechanisms responsible for ensuring the expression of one odorant receptor per olfactory sensory neuron.