PUBLICATION

Endothelial cilia mediate low flow sensing during zebrafish vascular development

Authors
Goetz, J.G., Steed, E., Ferreira, R.R., Roth, S., Ramspacher, C., Boselli, F., Charvin, G., Liebling, M., Wyart, C., Schwab, Y., Vermot, J.
ID
ZDB-PUB-140513-484
Date
2014
Source
Cell Reports   6: 799-808 (Journal)
Registered Authors
Liebling, Michael, Vermot, Julien, Wyart, Claire
Keywords
none
MeSH Terms
  • Neovascularization, Physiologic
  • Embryonic Development
  • Embryo, Nonmammalian/blood supply
  • Endothelial Cells/cytology
  • Endothelial Cells/ultrastructure
  • Animals
  • Zebrafish/embryology*
  • Cells, Cultured
  • Cardiovascular System/embryology*
  • Cilia/physiology
(all 10)
PubMed
24561257 Full text @ Cell Rep.
Abstract
The pattern of blood flow has long been thought to play a significant role in vascular morphogenesis, yet the flow-sensing mechanism that is involved at early embryonic stages, when flow forces are low, remains unclear. It has been proposed that endothelial cells use primary cilia to sense flow, but this has never been tested in vivo. Here we show, by noninvasive, high-resolution imaging of live zebrafish embryos, that endothelial cilia progressively deflect at the onset of blood flow and that the deflection angle correlates with calcium levels in endothelial cells. We demonstrate that alterations in shear stress, ciliogenesis, or expression of the calcium channel PKD2 impair the endothelial calcium level and both increase and perturb vascular morphogenesis. Altogether, these results demonstrate that endothelial cilia constitute a highly sensitive structure that permits the detection of low shear forces during vascular morphogenesis.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hsc5TgTransgenic Insertion
    is5TgTransgenic Insertion
      rk8TgTransgenic Insertion
        s1020tEtTransgenic Insertion
          tc300b
            		Point Mutation
            tc321
              		Point Mutation
              twu34TgTransgenic Insertion
                ubs3TgTransgenic Insertion
                  y7TgTransgenic Insertion
                    1 - 9 of 9
                    Show
                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    cmlc1MO2-cmlc1MRPHLNO
                    gata1aMO1-gata1aMRPHLNO
                    ift88MO1-ift88MRPHLNO
                    ift88MO3-ift88MRPHLNO
                    pkd2MO3-pkd2MRPHLNO
                    pkd2MO4-pkd2MRPHLNO
                    pkd2MO5-pkd2MRPHLNO
                    tnnt2aMO1-tnnt2aMRPHLNO
                    1 - 8 of 8
                    Show
                    Fish
                    Fish
                    hsc5Tg
                    is5Tg
                    pkd2tc321/tc321
                    rk8Tg
                    s1020tEt
                    tnnt2atc300b/tc300b
                    twu34Tg
                    ubs3Tg
                    y7Tg
                    1 - 9 of 9
                    Show
                    Antibodies
                    Name Type Antigen Genes Isotypes Host Organism
                    Ab1-pkd2polyclonalRabbit
                    1 - 1 of 1
                    Show
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    EGFPEFGEGFP
                    GAL4EFGGAL4
                    GAL4FFEFGGAL4FF
                    GFPEFGGFP
                    KaedeEFGKaede
                    mCherryEFGmCherry
                    1 - 6 of 6
                    Show
                    Mapping
                    No data available
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                    Citation
                    Goetz, J.G., Steed, E., Ferreira, R.R., Roth, S., Ramspacher, C., Boselli, F., Charvin, G., Liebling, M., Wyart, C., Schwab, Y., Vermot, J. (2014) Endothelial cilia mediate low flow sensing during zebrafish vascular development. Cell Reports. 6:799-808.