PUBLICATION
Oncogene-induced reactive oxygen species fuel hyperproliferation and DNA damage response activation
- Authors
- Ogrunc, M., Di Micco, R., Liontos, M., Bombardelli, L., Mione, M., Fumagalli, M., Gorgoulis, V.G., d'Adda di Fagagna, F.
- ID
- ZDB-PUB-140513-442
- Date
- 2014
- Source
- Cell death and differentiation 21: 998-1012 (Journal)
- Registered Authors
- Mione, Marina
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Cell Proliferation/drug effects
- DNA Damage/drug effects*
- DNA Damage/genetics
- Humans
- Mice
- NADPH Oxidases/genetics
- NADPH Oxidases/metabolism
- Oxidation-Reduction
- Oxidative Stress*
- Pancreatic Neoplasms/genetics*
- Pancreatic Neoplasms/pathology
- Reactive Oxygen Species/metabolism
- Reactive Oxygen Species/toxicity*
- Xenograft Model Antitumor Assays
- rac1 GTP-Binding Protein/genetics
- rac1 GTP-Binding Protein/metabolism
- PubMed
- 24583638 Full text @ Cell Death Differ.
Citation
Ogrunc, M., Di Micco, R., Liontos, M., Bombardelli, L., Mione, M., Fumagalli, M., Gorgoulis, V.G., d'Adda di Fagagna, F. (2014) Oncogene-induced reactive oxygen species fuel hyperproliferation and DNA damage response activation. Cell death and differentiation. 21:998-1012.
Abstract
Oncogene-induced reactive oxygen species (ROS) have been proposed to be signaling molecules that mediate proliferative cues. However, ROS may also cause DNA damage and proliferative arrest. How these apparently opposite roles can be reconciled, especially in the context of oncogene-induced cellular senescence, which is associated both with aberrant mitogenic signaling and DNA damage response (DDR)-mediated arrest, is unclear. Here, we show that ROS are indeed mitogenic signaling molecules that fuel oncogene-driven aberrant cell proliferation. However, by their very same ability to mediate cell hyperproliferation, ROS eventually cause DDR activation. We also show that oncogenic Ras-induced ROS are produced in a Rac1 and NADPH oxidase (Nox4)-dependent manner. In addition, we show that Ras-induced ROS can be detected and modulated in a living transparent animal: the zebrafish. Finally, in cancer we show that Nox4 is increased in both human tumors and a mouse model of pancreatic cancer and specific Nox4 small-molecule inhibitors act synergistically with existing chemotherapic agents.
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
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Orthology
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