PUBLICATION

Protective role of edaravone against neomycin-induced ototoxicity in zebrafish

Authors
Choi, J., Chang, J., Jun, H.J., Im, G.J., Chae, S.W., Lee, S.H., Kwon, S.Y., Jung, H.H., Chung, A.Y., Park, H.C.
ID
ZDB-PUB-140513-41
Date
2014
Source
Journal of applied toxicology : JAT   34: 554-61 (Journal)
Registered Authors
Chung, Ah-Young, Park, Hae-Chul
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Antipyrine/analogs & derivatives*
  • Antipyrine/pharmacology
  • Apoptosis/drug effects
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/ultrastructure
  • Hair Cells, Auditory/drug effects*
  • Hair Cells, Auditory/ultrastructure
  • In Situ Nick-End Labeling
  • Microscopy, Confocal
  • Microscopy, Electron, Scanning
  • Microscopy, Electron, Transmission
  • Mitochondria/drug effects
  • Mitochondria/ultrastructure
  • Neomycin/toxicity*
  • Neuroprotective Agents/pharmacology*
  • Zebrafish*/embryology
  • Zebrafish*/genetics
PubMed
24795994 Full text @ J. Appl. Toxicol.
Abstract
Aminoglycosides such as neomycin are one of the most commonly prescribed types of antibiotics worldwide. However, these drugs appear to generate free radicals within the inner ear, which can result in permanent hearing loss. We evaluated the effects of edaravone, a neuroprotective agent, on neomycin-induced ototoxicity in transgenic zebrafish. The 5-day post fertilization (dpf) zebrafish larvae were exposed to 125 μM neomycin and various concentrations of edaravone for 1 h. Hair cell survival was calculated as average numbers of the hair cells in the control group, which was not exposed to neomycin. Ultrastructural changes were evaluated using a scanning electron microscope (SEM) and transmission electron microscope (TEM). Edaravone protected against neomycin-induced hair cell loss in the neuromasts (1000 μM: 11.6 ± 1.1 cells, neomycin only: 5.5 ± 0.5 cells; n = 10, P<0.05) and decreased the TUNEL reaction for detecting apoptosis. In ultrastructural analysis, structures of mitochondria and hair cells within neuromasts were preserved in zebrafish exposed to 125 μM neomycin and 1000 μM edaravone for 1 h. Edaravone protected against neomycin-induced hair cell loss by preventing apoptosis.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes