ZFIN ID: ZDB-PUB-140513-397
Inter-cellular exchange of cellular components via VE-cadherin-dependent trans-endocytosis
Sakurai, T., Woolls, M.J., Jin, S.W., Murakami, M., Simons, M.
Date: 2014
Source: PLoS One   9: e90736 (Journal)
Registered Authors: Jin, Suk-Won, Simons, Michael, Woolls, Melissa
Keywords: none
MeSH Terms:
  • Actins/metabolism
  • Animals
  • Antigens, CD/metabolism*
  • COS Cells
  • Cadherins/metabolism*
  • Cell Communication
  • Chlorocebus aethiops
  • Coculture Techniques
  • Endocytosis*
  • Human Umbilical Vein Endothelial Cells/metabolism*
  • Humans
  • Myosins/metabolism
  • Protein Transport
  • Vinculin/metabolism
  • Zebrafish
  • rac1 GTP-Binding Protein/metabolism
PubMed: 24603875 Full text @ PLoS One
Cell-cell communications typically involve receptor-mediated signaling initiated by soluble or cell-bound ligands. Here, we report a unique mode of endocytosis: proteins originating from cell-cell junctions and cytosolic cellular components from the neighboring cell are internalized, leading to direct exchange of cellular components between two adjacent endothelial cells. VE-cadherins form transcellular bridges between two endothelial cells that are the basis of adherence junctions. At such adherens junction sites, we observed the movement of the entire VE-cadherin molecule from one endothelial cell into the other with junctional and cytoplasmic components. This phenomenon, here termed trans-endocytosis, requires the establishment of a VE-cadherin homodimer in trans with internalization proceeding in a Rac1-, and actomyosin-dependent manner. Importantly, the trans-endocytosis is not dependent on any known endocytic pathway including clathrin-dependent endocytosis, macropinocytosis or phagocytosis. This novel form of cell-cell communications, leading to a direct exchange of cellular components, was observed in 2D and 3D-cultured endothelial cells as well as in the developing zebrafish vasculature.