|ZFIN ID: ZDB-PUB-140513-397|
Inter-cellular exchange of cellular components via VE-cadherin-dependent trans-endocytosis
Sakurai, T., Woolls, M.J., Jin, S.W., Murakami, M., Simons, M.
|Source:||PLoS One 9: e90736 (Journal)|
|Registered Authors:||Jin, Suk-Won, Simons, Michael, Woolls, Melissa|
|PubMed:||24603875 Full text @ PLoS One|
Sakurai, T., Woolls, M.J., Jin, S.W., Murakami, M., Simons, M. (2014) Inter-cellular exchange of cellular components via VE-cadherin-dependent trans-endocytosis. PLoS One. 9:e90736.
ABSTRACTCell-cell communications typically involve receptor-mediated signaling initiated by soluble or cell-bound ligands. Here, we report a unique mode of endocytosis: proteins originating from cell-cell junctions and cytosolic cellular components from the neighboring cell are internalized, leading to direct exchange of cellular components between two adjacent endothelial cells. VE-cadherins form transcellular bridges between two endothelial cells that are the basis of adherence junctions. At such adherens junction sites, we observed the movement of the entire VE-cadherin molecule from one endothelial cell into the other with junctional and cytoplasmic components. This phenomenon, here termed trans-endocytosis, requires the establishment of a VE-cadherin homodimer in trans with internalization proceeding in a Rac1-, and actomyosin-dependent manner. Importantly, the trans-endocytosis is not dependent on any known endocytic pathway including clathrin-dependent endocytosis, macropinocytosis or phagocytosis. This novel form of cell-cell communications, leading to a direct exchange of cellular components, was observed in 2D and 3D-cultured endothelial cells as well as in the developing zebrafish vasculature.