PUBLICATION
Inter-cellular exchange of cellular components via VE-cadherin-dependent trans-endocytosis
- Authors
- Sakurai, T., Woolls, M.J., Jin, S.W., Murakami, M., Simons, M.
- ID
- ZDB-PUB-140513-397
- Date
- 2014
- Source
- PLoS One 9: e90736 (Journal)
- Registered Authors
- Jin, Suk-Won, Simons, Michael, Woolls, Melissa
- Keywords
- none
- MeSH Terms
-
- rac1 GTP-Binding Protein/metabolism
- Cell Communication
- Actins/metabolism
- Chlorocebus aethiops
- Endocytosis*
- Coculture Techniques
- Vinculin/metabolism
- Animals
- Cadherins/metabolism*
- Antigens, CD/metabolism*
- Human Umbilical Vein Endothelial Cells/metabolism*
- COS Cells
- Protein Transport
- Myosins/metabolism
- Zebrafish
- Humans
- PubMed
- 24603875 Full text @ PLoS One
Citation
Sakurai, T., Woolls, M.J., Jin, S.W., Murakami, M., Simons, M. (2014) Inter-cellular exchange of cellular components via VE-cadherin-dependent trans-endocytosis. PLoS One. 9:e90736.
Abstract
Cell-cell communications typically involve receptor-mediated signaling initiated by soluble or cell-bound ligands. Here, we report a unique mode of endocytosis: proteins originating from cell-cell junctions and cytosolic cellular components from the neighboring cell are internalized, leading to direct exchange of cellular components between two adjacent endothelial cells. VE-cadherins form transcellular bridges between two endothelial cells that are the basis of adherence junctions. At such adherens junction sites, we observed the movement of the entire VE-cadherin molecule from one endothelial cell into the other with junctional and cytoplasmic components. This phenomenon, here termed trans-endocytosis, requires the establishment of a VE-cadherin homodimer in trans with internalization proceeding in a Rac1-, and actomyosin-dependent manner. Importantly, the trans-endocytosis is not dependent on any known endocytic pathway including clathrin-dependent endocytosis, macropinocytosis or phagocytosis. This novel form of cell-cell communications, leading to a direct exchange of cellular components, was observed in 2D and 3D-cultured endothelial cells as well as in the developing zebrafish vasculature.
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Human Disease / Model
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