ZFIN ID: ZDB-PUB-140513-335
Multilayer mounting for long-term light sheet microscopy of zebrafish
Weber, M., Mickoleit, M., Huisken, J.
Date: 2014
Source: Journal of visualized experiments : JoVE   (84): e51119 (Journal)
Registered Authors: Huisken, Jan, Mickoleit, Michaela
Keywords: none
MeSH Terms:
  • Animals
  • Female
  • Male
  • Microscopy/instrumentation*
  • Microscopy/methods*
  • Sepharose
  • Time-Lapse Imaging/instrumentation*
  • Time-Lapse Imaging/methods*
  • Zebrafish/embryology*
PubMed: 24637614 Full text @ J. Vis. Exp.
Light sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is particularly suited for long-term time-lapse imaging over many hours up to several days. However, an appropriate sample mounting strategy is needed that offers both confinement and normal development of the sample. Multilayer mounting, a new embedding technique using low-concentration agarose in optically clear tubes, now overcomes this limitation and unleashes the full potential of light sheet microscopy for real-time developmental biology.