|ZFIN ID: ZDB-PUB-140513-335|
Multilayer mounting for long-term light sheet microscopy of zebrafish
Weber, M., Mickoleit, M., Huisken, J.
|Source:||Journal of visualized experiments : JoVE (84): e51119 (Journal)|
|Registered Authors:||Huisken, Jan, Mickoleit, Michaela|
|PubMed:||24637614 Full text @ J. Vis. Exp.|
Weber, M., Mickoleit, M., Huisken, J. (2014) Multilayer mounting for long-term light sheet microscopy of zebrafish. Journal of visualized experiments : JoVE. (84):e51119.
ABSTRACTLight sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is particularly suited for long-term time-lapse imaging over many hours up to several days. However, an appropriate sample mounting strategy is needed that offers both confinement and normal development of the sample. Multilayer mounting, a new embedding technique using low-concentration agarose in optically clear tubes, now overcomes this limitation and unleashes the full potential of light sheet microscopy for real-time developmental biology.