PUBLICATION

Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos

Authors

Qiao, L., Gao, H., Zhang, T., Jing, L., Xiao, C., Xiao, Y., Luo, N., Zhu, H., Meng, W., Xu, H., Mo, X.

ID

ZDB-PUB-140513-279

Date

2014

Source

Scientific Reports 4: 4470 (Journal)

Registered Authors

Mo, Xianming, Zhang, Ting

Keywords

none

MeSH Terms

Morphogenesis/genetics
Cell Movement/genetics
Myocardium/cytology
Embryo, Nonmammalian
Integrin alpha5/genetics
Integrin alpha5/metabolism*
Zebrafish
Zebrafish Proteins/antagonists & inhibitors
Zebrafish Proteins/genetics*
Heart/growth & development
Animals
Embryonic Development*
Transcription Factors/antagonists & inhibitors
Transcription Factors/genetics*
Fibronectins/genetics
Fibronectins/metabolism*

(all 16)

PubMed

24667151 Full text @ Sci. Rep.

Abstract

The Snail family member snail encodes a zinc finger-containing transcriptional factor that is involved in heart formation. Yet, little is known about how Snail regulates heart development. Here, we identified that one of the duplicated snail genes, snai1b, was expressed in the heart region of zebrafish embryos. Depletion of Snai1b function dramatically reduced expression of α5 integrin, disrupted Fibronectin layer in the heart region, especially at the midline, and prevented migration of cardiac precursors, resulting in defects in cardiac morphology and function in zebrafish embryos. Injection of α5β1 protein rescued the Fibronectin layer and then the myocardial precursor migration in snai1b knockdown embryos. The results provide the molecular mechanism how Snail controls the morphogenesis of heart during embryonic development.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
EGFP	s843Tg	control	Long-pec to Protruding-mouth	endocardium	IFL	Fig. 1
EGFP	s843Tg + MO1-snai1b	standard conditions	Long-pec to Protruding-mouth	endocardium	IFL	Fig. 1
EGFP	twu34Tg	control	Protruding-mouth	atrium	IFL	Fig. 1
EGFP	twu34Tg	control	Long-pec	atrium	IFL	Fig. 1
EGFP	twu34Tg	control	Long-pec	cardiac ventricle	IFL	Fig. 1
EGFP	twu34Tg	control	Protruding-mouth	cardiac ventricle	IFL	Fig. 1
EGFP	twu34Tg	control	Protruding-mouth	heart	IFL	Fig. 1
EGFP	twu34Tg	control	Long-pec	heart	IFL	Fig. 1
EGFP	twu34Tg	control	14-19 somites	myocardial precursor	IHC	Fig. 3
EGFP	twu34Tg + MO1-snai1b	standard conditions	Long-pec	atrium	IFL	Fig. 1

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-snai1b	standard conditions	10-13 somites	cardiac muscle progenitor cell migration to the midline involved in heart field formation delayed, abnormal	Fig. 2
AB + MO1-snai1b	standard conditions	10-13 somites	cardiac muscle progenitor cell migration to the midline involved in heart field formation process quality, abnormal	Fig. 2
AB + MO1-snai1b	standard conditions	10-13 somites	cardioblast cell midline fusion delayed, abnormal	Fig. 2
AB + MO1-snai1b	standard conditions	10-13 somites	cell migration to the midline involved in heart development delayed, abnormal	Fig. 2
AB + MO1-snai1b	standard conditions	10-13 somites	cell migration to the midline involved in heart development process quality, abnormal	Fig. 2
AB + MO1-snai1b	standard conditions	14-19 somites	cardiac muscle progenitor cell migration to the midline involved in heart field formation delayed, abnormal	Fig. 2
AB + MO1-snai1b	standard conditions	14-19 somites	cardiac muscle progenitor cell migration to the midline involved in heart field formation process quality, abnormal	Fig. 2
AB + MO1-snai1b	standard conditions	14-19 somites	cardioblast cell midline fusion delayed, abnormal	Fig. 2
AB + MO1-snai1b	standard conditions	14-19 somites	cell migration to the midline involved in heart development delayed, abnormal	Fig. 2
AB + MO1-snai1b	standard conditions	14-19 somites	cell migration to the midline involved in heart development process quality, abnormal	Fig. 2

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
s843Tg	Tg(kdrl:EGFP)	Transgenic Insertion
twu34Tg	Tg(myl7:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
snai1b	MO1-snai1b	MRPHLNO
tp53	MO4-tp53	MRPHLNO

Fish

Fish
AB
AB + MO1-snai1b
s843Tg
s843Tg + MO1-snai1b
twu34Tg
twu34Tg + MO1-snai1b

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab2-fn	polyclonal			Rabbit

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Qiao et al., 2014 ZDB-PUB-140513-279 PMID:24667151

Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos

Qiao et al., 2014

ZDB-PUB-140513-279
PMID:24667151