|ZFIN ID: ZDB-PUB-140513-171|
In Vivo Optogenetics for Light-Induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein
Teh, C., Korzh, V.
|Source:||Methods in molecular biology (Clifton, N.J.) 1148: 229-38 (Chapter)|
|Registered Authors:||Korzh, Vladimir, Teh, Cathleen|
|PubMed:||24718805 Full text @ Meth. Mol. Biol.|
Teh, C., Korzh, V. (2014) In Vivo Optogenetics for Light-Induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein. Methods in molecular biology (Clifton, N.J.). 1148:229-38.
ABSTRACTOptogenetic methods are gaining broad recognition. The zebrafish is particularly useful for these applications as a model vertebrate due to a unique combination of translucent embryos/larvae and efficient transgenesis. Here, we describe a zebrafish model of light-induced cardiac deficiency. Upon illumination with intense green light, the membrane-tethered photosensitizer protein KillerRed acts as a photoinducer of reactive oxygen species which in turn cause changes in heart rate and contractility in hearts that express this transgene.
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