ZFIN ID: ZDB-PUB-140513-171
In Vivo Optogenetics for Light-Induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein
Teh, C., Korzh, V.
Date: 2014
Source: Methods in molecular biology (Clifton, N.J.)   1148: 229-38 (Chapter)
Registered Authors: Korzh, Vladimir, Teh, Cathleen
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Female
  • Gene Expression
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Heart Rate
  • Larva/genetics
  • Larva/metabolism
  • Larva/radiation effects
  • Light
  • Male
  • Myocardial Contraction
  • Myocardium/metabolism
  • Myocardium/pathology
  • Optogenetics*
  • Oxidative Stress/radiation effects*
  • Photosensitizing Agents/metabolism
  • Ventricular Function
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed: 24718805 Full text @ Meth. Mol. Biol.
Optogenetic methods are gaining broad recognition. The zebrafish is particularly useful for these applications as a model vertebrate due to a unique combination of translucent embryos/larvae and efficient transgenesis. Here, we describe a zebrafish model of light-induced cardiac deficiency. Upon illumination with intense green light, the membrane-tethered photosensitizer protein KillerRed acts as a photoinducer of reactive oxygen species which in turn cause changes in heart rate and contractility in hearts that express this transgene.