Proteome-Driven Elucidation of Adaptive Responses to Combined Vitamin E and C Deficiency in Zebrafish
- Authors
- Motorykin, I., Traber, M.G., Tanguay, R.L., and Maier, C.S.
- ID
- ZDB-PUB-140404-1
- Date
- 2014
- Source
- Journal of Proteome Research 13(3): 1647-56 (Journal)
- Registered Authors
- Tanguay, Robyn L.
- Keywords
- none
- MeSH Terms
-
- Adaptation, Physiological*
- Animals
- Ascorbic Acid/administration & dosage
- Ascorbic Acid/metabolism*
- Ascorbic Acid Deficiency/metabolism*
- Chromatography, Liquid
- Humans
- Mass Spectrometry/methods
- Metabolic Networks and Pathways
- Protein Interaction Mapping
- Proteome/analysis*
- Proteome/metabolism
- Tissue Extracts/chemistry
- Vitamin E/administration & dosage
- Vitamin E/metabolism*
- Vitamin E Deficiency/metabolism*
- Zebrafish
- PubMed
- 24476500 Full text @ J. Proteome Res.
The purpose of this study was to determine the system-wide consequences of deficiencies in two essential micronutrients, vitamins E and C, on the proteome using zebrafish (Danio rerio) as one of the few vertebrate models that similar to humans cannot synthesize vitamin C. We describe a label-free proteomics workflow to detect changes in protein abundance estimates dependent on vitamin regimes. We used ion-mobility-enhanced data-independent tandem mass spectrometry to determine differential regulation of proteins in response to low dietary levels of vitamin C with or without vitamin E. The detection limit of the method was as low as 20 amol, and the dynamic range was five orders of magnitude for the protein-level estimates. On the basis of the quantitative changes obtained, we built a network of protein interactions that reflect the whole organism’s response to vitamin C deficiency. The proteomics-driven study revealed that in vitamin-E-deficient fish, vitamin C deficiency is associated with induction of stress response, astrogliosis, and a shift from glycolysis to glutaminolysis as an alternative mechanism to satisfy cellular energy requirements.