Yossa, R., Sarker, P.K., Proulx, E., Saxena, V., Ekker, M., and Vandenburg, G.W. (2013) A Practical Approach for Sexing Zebrafish, Danio rerio. Journal of Applied Aquaculture. 25:148-153.
This study aimed at a simple, rapid, reliable, practical, and non-destructive
sexing technique for mature zebrafish (0.61 ± 0.011 g
each). In the first experiment, 36 fish of each sex were selected on
the basis of the presence of a prominent genital papilla on mature
females and bred randomly. All the couples laid fertilized eggs
with a mean fecundity of 304 eggs per female, for a 100% sexing
accuracy. In the second experiment, 140 mature zebrafish were
randomly selected and individually sexed like in the first experiment.
Each fish was then dissected in order to confirm the reliability
of this sexing technique through the observation of either the ovaries
or the testes. Upon dissection, an accuracy of 100% was recorded.
This study demonstrates that sexing mature zebrafish only on the
basis of the presence of a prominent genital papilla on the mature
female is a reliable technique.
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