Transcription of the SCL/TAL1 interrupting locus (Stil) is required for cell proliferation in adult zebrafish retinas
- Authors
- Sun, L., Li, P., Carr, A.L., Gorsuch, R., Yarka, C., Li, J., Bartlett, M., Pfister, D., Hyde, D.R., and Li, L.
- ID
- ZDB-PUB-140402-9
- Date
- 2014
- Source
- The Journal of biological chemistry 289(10): 6934-40 (Journal)
- Registered Authors
- Gorsuch, Ryne, Hyde, David R., Li, Lei
- Keywords
- none
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/physiology*
- Cell Proliferation*
- Ependymoglial Cells/drug effects
- Ependymoglial Cells/metabolism
- Ependymoglial Cells/pathology
- Gene Expression
- Gene Knockdown Techniques
- Hedgehog Proteins/genetics
- Hedgehog Proteins/metabolism*
- Oxidopamine/pharmacology
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/physiology*
- Repressor Proteins/genetics
- Retina/drug effects
- Retina/metabolism
- Retina/pathology*
- Retinal Degeneration/chemically induced
- Retinal Degeneration/pathology
- Retinal Rod Photoreceptor Cells/drug effects
- Retinal Rod Photoreceptor Cells/metabolism
- Retinal Rod Photoreceptor Cells/pathology
- Signal Transduction
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/physiology*
- PubMed
- 24469449 Full text @ J. Biol. Chem.
The human oncogene SCL/TAL1 interrupting locus (Stil) is highly conserved in vertebrate species. Previously, we identified a homolog of the Stil gene in zebrafish mutant (night blindness b, nbb), which showed neural defects in the retina (e.g. dopaminergic cell degeneration and/or lack of regeneration). In this research, we examined the roles of Stil in cell proliferation after degeneration in adult zebrafish retinas. We demonstrated that knockdown of Stil gene expression or inhibition of Sonic hedgehog (Shh) signaling transduction decreases the rate of cell proliferation. In contrast, activation of Shh signal transduction promotes cell proliferation. In nbb+/ retinas, inhibition of SUFU (a repressor in the Shh pathway) rescues the defects in cell proliferation due to down-regulation of Stil gene expression. The latter data suggest that Stil play a role in cell proliferation through the Shh signal transduction pathway.