PUBLICATION

Early Development and Tissue Distribution of Pseudoloma neurophilia in the Zebrafish, Danio rerio

Authors
Sanders, J.L., Peterson, T.S., and Kent, M.L.
ID
ZDB-PUB-140317-17
Date
2014
Source
The Journal of eukaryotic microbiology   61(3): 238-46 (Journal)
Registered Authors
Kent, Michael
Keywords
histology, in situ hybridization, infection, microsporidia
MeSH Terms
  • Animals
  • Histocytochemistry
  • In Situ Hybridization
  • Microsporidia/cytology
  • Microsporidia/genetics
  • Microsporidia/growth & development*
  • Microsporidia/isolation & purification*
  • RNA, Ribosomal, 18S/genetics
  • Spores, Protozoan/cytology
  • Spores, Protozoan/isolation & purification
  • Zebrafish/anatomy & histology
  • Zebrafish/parasitology*
PubMed
24411000 Full text @ J. Eukaryot. Microbiol.
Abstract

The early proliferative stages of the microsporidian parasite, Pseudoloma neurophilia were visualized in larval zebrafish, Danio rerio, using histological sections with a combination of an in situ hybridization probe specific to the P. neurophilia small-subunit ribosomal RNA gene, standard hematoxylin-eosin stain, and the Luna stain to visualize spores. Beginning at 5 d post fertilization, fish were exposed to P. neurophilia and examined at 12, 24, 36, 48, 72, 96, and 120 h post exposure (hpe). At 12 hpe, intact spores in the intestinal lumen and proliferative stages developing in the epithelial cells of the anterior intestine and the pharynx and within hepatocytes were observed. Proliferative stages were visualized in the pancreas and kidney at 36-48 hpe and in the spinal cord, eye, and skeletal muscle beginning at 72 hpe. The first spore stages of P. neurophilia were observed at 96 hpe in the pharyngeal epithelium, liver, spinal cord, and skeletal muscle. The parasite was only observed in the brain of larval fish at 120 hpe. The distribution of the early stages of P. neurophilia and the lack of mature spores until 96 hpe indicates that the parasite gains access to organs distant from the initial site of entry, likely by penetrating the intestinal wall with the polar tube.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes