Msd1/SSX2IP-dependent microtubule anchorage ensures spindle orientation and primary cilia formation
- Authors
- Hori, A., Ikebe, C., Tada, M., and Toda, T.
- ID
- ZDB-PUB-140303-35
- Date
- 2014
- Source
- EMBO reports 15(2): 175-184 (Journal)
- Registered Authors
- Tada, Masazumi
- Keywords
- none
- MeSH Terms
-
- Animals
- Cilia/metabolism
- HeLa Cells
- Humans
- Microtubule-Associated Proteins/genetics
- Microtubule-Associated Proteins/metabolism*
- Microtubules/metabolism*
- Neoplasm Proteins/genetics
- Neoplasm Proteins/metabolism*
- Repressor Proteins/genetics
- Repressor Proteins/metabolism*
- Spindle Apparatus/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 24397932 Full text @ EMBO Rep.
Anchoring microtubules to the centrosome is critical for cell geometry and polarity, yet the molecular mechanism remains unknown. Here we show that the conserved human Msd1/SSX2IP is required for microtubule anchoring. hMsd1/SSX2IP is delivered to the centrosome in a centriolar satellite-dependent manner and binds the microtubule-nucleator γ-tubulin complex. hMsd1/SSX2IP depletion leads to disorganised interphase microtubules and misoriented mitotic spindles with reduced length and intensity. Furthermore, hMsd1/SSX2IP is essential for ciliogenesis, and during zebrafish embryogenesis, knockdown of its orthologue results in ciliary defects and disturbs left-right asymmetry. We propose that the Msd1 family comprises conserved microtubule-anchoring proteins.