PUBLICATION
A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies
- Authors
- Wilkinson, R.N., Elworthy, S., Ingham, P.W., and van Eeden, F.J.
- ID
- ZDB-PUB-140213-17
- Date
- 2013
- Source
- Biotechniques 55(6): 314-316 (Journal)
- Registered Authors
- Elworthy, Stone, Ingham, Philip, van Eeden, Freek, Wilkinson, Robert
- Keywords
- none
- MeSH Terms
-
- Animals
- Biopsy/veterinary
- Genotyping Techniques*
- High-Throughput Nucleotide Sequencing
- Larva/genetics
- Polymerase Chain Reaction/methods
- Tail/pathology
- Zebrafish/genetics*
- Zebrafish/growth & development
- PubMed
- 24344681 Full text @ Biotechniques
Citation
Wilkinson, R.N., Elworthy, S., Ingham, P.W., and van Eeden, F.J. (2013) A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies. Biotechniques. 55(6):314-316.
Abstract
Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping