ZFIN ID: ZDB-PUB-140213-17
A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies
Wilkinson, R.N., Elworthy, S., Ingham, P.W., and van Eeden, F.J.
Date: 2013
Source: Biotechniques   55(6): 314-316 (Journal)
Registered Authors: Elworthy, Stone, Ingham, Philip, van Eeden, Freek, Wilkinson, Robert
Keywords: none
MeSH Terms:
  • Animals
  • Biopsy/veterinary
  • Genotyping Techniques*
  • High-Throughput Nucleotide Sequencing
  • Larva/genetics
  • Polymerase Chain Reaction/methods
  • Tail/pathology
  • Zebrafish/genetics*
  • Zebrafish/growth & development
PubMed: 24344681 Full text @ Biotechniques

Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.