| ZFIN ID: ZDB-PUB-140123-26 |
Analysis of embryonic and larval zebrafish skeletal myofibers from dissociated preparations
Horstick, E.J., Gibbs, E.M., Li, X., Davidson, A.E., and Dowling, J.J.
| Date: | 2013 |
|---|---|
| Source: | Journal of visualized experiments : JoVE (81): e50259 (Journal) |
| Registered Authors: | Dowling, Jim |
| Keywords: | none |
| MeSH Terms: |
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| PubMed: | 24300240 Full text @ J. Vis. Exp. |
Citation
Horstick, E.J., Gibbs, E.M., Li, X., Davidson, A.E., and Dowling, J.J. (2013) Analysis of embryonic and larval zebrafish skeletal myofibers from dissociated preparations. Journal of visualized experiments : JoVE. (81):e50259.
ABSTRACT
Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.
ADDITIONAL INFORMATION
- Antibodies (2)
- Expression Data
- Fish (1)