PUBLICATION

Analysis of embryonic and larval zebrafish skeletal myofibers from dissociated preparations

Authors
Horstick, E.J., Gibbs, E.M., Li, X., Davidson, A.E., and Dowling, J.J.
ID
ZDB-PUB-140123-26
Date
2013
Source
Journal of visualized experiments : JoVE   (81): e50259 (Journal)
Registered Authors
Dowling, Jim
Keywords
none
MeSH Terms
  • Muscle Proteins/analysis
  • Muscle Proteins/metabolism
  • Immunohistochemistry
  • Muscle, Skeletal/chemistry
  • Muscle, Skeletal/cytology*
  • Muscle, Skeletal/metabolism
  • Muscle Fibers, Skeletal/chemistry
  • Muscle Fibers, Skeletal/cytology*
  • Muscle Fibers, Skeletal/metabolism
  • Larva
  • Zebrafish
  • Calcium/analysis
  • Calcium/metabolism
  • Animals
(all 14)
PubMed
24300240 Full text @ J. Vis. Exp.
Abstract

Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.

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Phenotype
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Horstick, E.J., Gibbs, E.M., Li, X., Davidson, A.E., and Dowling, J.J. (2013) Analysis of embryonic and larval zebrafish skeletal myofibers from dissociated preparations. Journal of visualized experiments : JoVE. (81):e50259.