PUBLICATION
Analysis of embryonic and larval zebrafish skeletal myofibers from dissociated preparations
- Authors
- Horstick, E.J., Gibbs, E.M., Li, X., Davidson, A.E., and Dowling, J.J.
- ID
- ZDB-PUB-140123-26
- Date
- 2013
- Source
- Journal of visualized experiments : JoVE (81): e50259 (Journal)
- Registered Authors
- Dowling, Jim
- Keywords
- none
- MeSH Terms
-
- Muscle Proteins/analysis
- Muscle Proteins/metabolism
- Muscle, Skeletal/chemistry
- Muscle, Skeletal/cytology*
- Muscle, Skeletal/metabolism
- Animals
- Larva
- Muscle Fibers, Skeletal/chemistry
- Muscle Fibers, Skeletal/cytology*
- Muscle Fibers, Skeletal/metabolism
- Calcium/analysis
- Calcium/metabolism
- Immunohistochemistry
- Zebrafish
- PubMed
- 24300240 Full text @ J. Vis. Exp.
Citation
Horstick, E.J., Gibbs, E.M., Li, X., Davidson, A.E., and Dowling, J.J. (2013) Analysis of embryonic and larval zebrafish skeletal myofibers from dissociated preparations. Journal of visualized experiments : JoVE. (81):e50259.
Abstract
Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping