ZFIN ID: ZDB-PUB-140123-11
Trb3 Regulates LR Axis formation in zebrafish embryos
Anuppalle, M., Maddirevula, S., Huh, T.L., and Rhee, M.
Date: 2013
Source: Molecules and cells   36(6): 542-547 (Journal)
Registered Authors: Huh, Tae-Lin
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Blastula/metabolism*
  • Body Patterning*
  • Cell Cycle Proteins/chemistry
  • Cell Cycle Proteins/genetics
  • Cell Cycle Proteins/metabolism*
  • Embryonic Development
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Heart/embryology
  • Liver/embryology
  • Sequence Alignment
  • Signal Transduction
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism
  • Ubiquitin-Protein Ligases/genetics
  • Ubiquitin-Protein Ligases/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 24292884 Full text @ Mol. Cells

Tribless family proteins are pseudokinases that lack DFG (Asp-Phe-Gly) motif in the functional kinase domain, regulating Akt and BMP pathways, insulin metabolism, hypoxia, and ubiquitination. This report concerns expression patterns and functional roles of trb3 in zebrafish embryonic development. trb3 is evolutionarily well-conserved and located on zebrafish chromosome 11. Spatiotemporal expression studies show that trb3 transcripts are abundant throughout embryogenesis, but confined to mesendodermal cells during the late blastula phase. Over-expression of trb3 ventralizes the embryos while a knockdown of trb3 using morpholino alters positioning of the heart, liver, and pancreatic buds as well as gut looping. Furthermore, constitutive activation of TGF-signaling with TARAM-A* (TGF—related type I receptor) significantly increases the level of trb3 transcripts during the late blastula phase. Over-expression of trb3 reduces the level of smurf1 transcripts, a member of TGF-signaling. We thus propose that Trb3 governs left-right (LR) axis patterning as a component of TGF-signaling in vertebrate embryonic development.