PUBLICATION
CXCR4 blockade and Sphingosine-1-phosphate activation facilitate engraftment of haematopoietic stem and progenitor cells in a non-myeloablative transplant model
- Authors
- Shayegi, N., Meyer, C., Lambert, K., Ehninger, G., Brand, M., and Bornhäuser, M.
- ID
- ZDB-PUB-131202-10
- Date
- 2014
- Source
- British journal of haematology 164(3): 409-13 (Journal)
- Registered Authors
- Brand, Michael
- Keywords
- engraftment, conditioning, zebrafish, AMD3100, SEW2871
- MeSH Terms
-
- Animal Experimentation
- Animals
- Hematopoietic Stem Cell Transplantation/methods*
- Hematopoietic Stem Cells/cytology*
- Hematopoietic Stem Cells/drug effects*
- Heterocyclic Compounds/pharmacology*
- Lysophospholipids/genetics
- Lysophospholipids/metabolism*
- Oxadiazoles/pharmacology*
- Receptors, CXCR4/antagonists & inhibitors*
- Receptors, CXCR4/genetics
- Receptors, CXCR4/metabolism
- Signal Transduction
- Sphingosine/analogs & derivatives*
- Sphingosine/genetics
- Sphingosine/metabolism
- Thiophenes/pharmacology*
- Transplantation Chimera
- Transplantation, Homologous
- Zebrafish
- PubMed
- 24180707 Full text @ Br. J. Haematol.
Citation
Shayegi, N., Meyer, C., Lambert, K., Ehninger, G., Brand, M., and Bornhäuser, M. (2014) CXCR4 blockade and Sphingosine-1-phosphate activation facilitate engraftment of haematopoietic stem and progenitor cells in a non-myeloablative transplant model. British journal of haematology. 164(3):409-13.
Abstract
Both immunosuppressive and cytoreductive effects of γ-irradiation contribute to engraftment of allogeneic haematopoietic stem and progenitor cells. We hypothesized that a release of host stem and progenitor cells from the niche prior to conditioning would permit engraftment after less intensive conditioning. Administration of AMD3100 and SEW2871 on days 4 to 2 followed by irradiation on day 1 in a non-myeloablative zebrafish transplant model resulted in a reduced radiation minimum dose of 10 Gy from 15 Gy being sufficient for engraftment. Targeting the SDF-1 (CXCL12)/CXCR4- and S1P/S1P1-axis increased the efficacy of allografting in an experimental transplant model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping