Varshney, G.K., and Burgess, S.M. (2014) Mutagenesis and phenotyping resources in zebrafish for studying development and human disease. Briefings in Functional Genomics. 13(2):82-94.
The zebrafish (Danio rerio) is an important model organism for studying development and human disease. The zebrafish has an excellent reference genome
and the functions of hundreds of genes have been tested using both forward and reverse genetic approaches. Recent years have
seen an increasing number of large-scale mutagenesis projects and the number of mutants or gene knockouts in zebrafish has
increased rapidly, including for the first time conditional knockout technologies. In addition, targeted mutagenesis techniques
such as zinc finger nucleases, transcription activator-like effector nucleases and clustered regularly interspaced short sequences
(CRISPR) or CRISPR-associated (Cas), have all been shown to effectively target zebrafish genes as well as the first reported
germline homologous recombination, further expanding the utility and power of zebrafish genetics. Given this explosion of
mutagenesis resources, it is now possible to perform systematic, high-throughput phenotype analysis of all zebrafish gene
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